Journal of Plant Biochemistry & Physiology
Open Access

Abstract

Standardization of an Efficient Protocol for Sterilization and Media for Direct Shoot Regeneration from Nodal Segments of Medicinal Herb Piper longum Linn

Ruby Tiwari

In the present research it was aimed to the development and standardization of an effective sterilization protocol and media for shoot regeneration technique for a medicinal herb Piper longum linn. Nodal explants from juvenile plants were cultured on Murashige and Skoog’s (MS) media with an array of different combinations of Cytokinins and Auxins. Among the various protocols tested for sterilization, the one containing 200 mg activated charcoal in 250 ml autoclaved water has found to be the best, for nodal segments and cultured in antibiotic containing media. Maximum growth were obtained from the media containing (MSL7) BAP(5 mg/l) NAA(0.5 mg/l) and (MSL10) BAP(3 mg/l)+IAA (0.5 mg/l) along with 15 mg Kanamycin. The findings of this study have to be used for development of an effective protocol for the sterilization and establishment of shoots from nodal explants. Best shoot regeneration was observed from nodal explants was used for further studies and micro shoots were transferred for rooting in IBA containing media and then go for acclimatization and hardening.