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ISSN: 2329-9029

Journal of Plant Biochemistry & Physiology
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Research Article

Standardization of an Efficient Protocol for Sterilization and Media for Direct Shoot Regeneration from Nodal Segments of Medicinal Herb Piper longum Linn

Ruby Tiwari*
Department of Biotechnology, IMS Engineering College, India
*Corresponding Author : Ruby Tiwari
Department of Biotechnology
IMS Engineering College
NH-24, Near Dasna
Ghaziabad, UP, India
[email protected]
Received January 06, 2016; Accepted February 03, 2016; Published February 09, 2016
Citation: Tiwari R (2016) Standardization of an Efficient Protocol for Sterilization and Media for Direct Shoot Regeneration from Nodal Segments of Medicinal Herb Piper longum Linn. J Plant Biochem Physiol 4:163. doi:10.4172/2329-9029.1000163
Copyright: © 2016 Tiwari R. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


In the present research it was aimed to the development and standardization of an effective sterilization protocol and media for shoot regeneration technique for a medicinal herb Piper longum linn. Nodal explants from juvenile plants were cultured on Murashige and Skoog’s (MS) media with an array of different combinations of Cytokinins and Auxins. Among the various protocols tested for sterilization, the one containing 200 mg activated charcoal in 250 ml autoclaved water has found to be the best, for nodal segments and cultured in antibiotic containing media. Maximum growth were obtained from the media containing (MSL7) BAP(5 mg/l) NAA(0.5 mg/l) and (MSL10) BAP(3 mg/l)+IAA (0.5 mg/l) along with 15 mg Kanamycin. The findings of this study have to be used for development of an effective protocol for the sterilization and establishment of shoots from nodal explants. Best shoot regeneration was observed from nodal explants was used for further studies and micro shoots were transferred for rooting in IBA containing media and then go for acclimatization and hardening.


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