alexa Statistically Optimized Synthesis of Silver Nanocubes from Peel Extracts of Citrus limetta and Potential Application in Waste Water Treatment
ISSN: 1948-5948

Journal of Microbial & Biochemical Technology
Open Access

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Research Article

Statistically Optimized Synthesis of Silver Nanocubes from Peel Extracts of Citrus limetta and Potential Application in Waste Water Treatment

Priyanka Trivedi, Manish Khandelwal and Priyanka Srivastava*

School of Bio Sciences and Technology, Vellore Institute of Technology University, Tamil Nadu, India

*Corresponding Author:
Priyanka Srivastava
Assistant Professor, Division of Biomedical Sciences
School of Bio Sciences and Technology, VIT University
Vellore 632014, Tamil Nadu, India
Tel: +91 8870514196
E-mail: [email protected]

Published date: July 11, 2014; Published date: August 27, 2014; Published date: September 02, 2014

Citation: Trivedi P, Khandelwal M, Srivastava P (2014) Statistically Optimized Synthesis of Silver Nanocubes from Peel Extracts of Citrus limetta and Potential Application in Waste Water Treatment. J Microb Biochem Technol S4:004. doi:10.4172/1948-5948.S4-004

Copyright: © 2014 Trivedi P, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited



This work reports statistically optimized synthesis of silver nanocubes from cost effective, readily available bioreductant, in the form of peels of Citrus limetta. Effect of six variables viz. temperature, pH, volume of reductant, reaction volume, illumination, concentration of silver nitrate was examined using Placket Burman Design. Temperature, pH and illumination were found to significantly affect the shape and size of silver nanoparticles. Best conditions for the synthesis of silver nanocubes were 0.001 M AgNO3 with temperature 27°C and pH 6 under dark incubation. Characterization of the synthesized nanocubes was done using UV-Vis spectroscopy, Scanning Electron Microscopy (SEM). SEM analysis showed clear synthesis of nanocubes in the range of 37-59 nm to 163-205 nm. Additionally, an investigation of biologically synthesized nanocubes in wastewater treatment was done. A filter was designed. The colloidal solution of silver nanoparticles was mixed with activated charcoal in ratio of 1:1, 1:2 and 2:1 respectively, to make filter bed. A series of microbiological tests carried out on the filtrates obtained, showed that the microbes were killed to an extent of 97%, especially E. coli. Our work indicates that silver nanoparticle loaded activated charcoal filter can be used as an excellent antibacterial water filter.


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