Stock Structure Delineation of the African Catfish (Clarius gariepinus) in Selected Populations in Kenya Using Mitochondrial DNA (Dloop) Variability
Cynthia Nyunja1*, Joyce Maina1, Joshua Amimo1, Felix Kibegwa1, David Harper1,2 and Joseph Jung’a1
1Department of Animal Production, University of Nairobi, Kenya
2University of Leicester, England
- *Corresponding Author:
- Cynthia Nyunja
Department of Animal Production
University of Nairobi, Kenya
Tel: +254 20 318262
E-mail: [email protected]
Received Date: June 09, 2017; Accepted Date: June 22, 2017; Published Date: June 24, 2017
Citation: Nyunja C, Maina J, Amimo J, Kibegwa F, Harper D, et al. (2017) Stock Structure Delineation of the African Catfish (Clarius gariepinus) in Selected Populations in Kenya Using Mitochondrial DNA (Dloop) Variability. J Aquac Res Development 8:485. doi: 10.4172/2155-9546.1000485
Copyright: © 2017 Nyunja C, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
This study genetically characterized five populations of the African catfish (Clarius gariepinus) in Kenya. Samples were obtained from five sites in the country–Athi River hatchery, Kisii Fingerling Production Centre (FPC), Jewlett hatchery, Sagana Hatchery Station and Lake Baringo. DNA was extracted from tissue samples, followed by amplification and sequencing of the dloop region. Haplotype diversities, phylogenetic structure and variation at the dloop region of mitochondrial DNA were assessed.
Mitochondrial DNA analyses indicated that the sampled species showed genetic diversity between its populations. The genetic results were congruent indicating the differences in diversities and haplotype similarities of catfish samples from different sites. The Sagana, Kisii FPC, Jewlett and Baringo population cluster overlapped indicating possibly shared source of brood stock. The Athi river population was in a different cluster and its distinctiveness is attributed to imported brood stock. Both Athi River hatchery and Lake Baringo populations were highly variable and has great potential for production.