STR Profiling of Human Cell Lines: Challenges and Possible Solutions to the Growing Problem
- *Corresponding Author:
- Dr. Manohar R Furtado, PhD
Life Technologies 850 Lincoln
Foster City, CA 94404, USA
Tel: 650 554 2670
Fax: 650 554 6795
E-mail: [email protected]
Received Date: September 09, 2011; Accepted Date: October 10, 2011; Published Date: October 19, 2011
Citation: Fang R, Shewale JG, Nguyen VT, Cardoso H, Swerdel M, et al. (2011) STR Profiling of Human Cell Lines: Challenges and Possible Solutions to the Growing Problem. J Forensic Res S2:005. doi: 10.4172/2157-7145.S2-005
Copyright: © 2011 Fang R, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Genomic DNA preparations from 60 human cell lines from the National Cancer Institute (NCI), 48 human cell lines from American Type Culture Collection (ATCC) and 19 embryonic cell lines were profiled for autosomal short tandem repeat (STR) loci using the AmpFÃ¢ÂÂSTR ® Identifiler ® kit. Each DNA sample was profiled at least twice to ensure consistency and reproducibility of results. The resulting STR profiles were compared with the STR profiles in the database of ATCC. The allele calls for the common loci between the Identifiler ® kit and the database were identical except for one DNA sample, which we attribute to amplification artifacts. We have observed a high percentage of the STR loci exhibiting allelic imbalance. Certain STR loci for some cell lines exhibited 3 or more alleles. This type of observation can result from a unique profile for a given cell line or as the result of clonotypic heterozygosity and is not necessarily due to contamination. Our study demonstrates that STR based technologies are useful for cell line authentication applications. These data, combined with data from other researchers, will enable the development of a standard genotyping protocol for cell line authentication.