alexa Stress during Lactation Affects Fatty Acid Amide Hydrolase Protein Expression in Adipose Tissue and Liver of Adult Mice
ISSN: 2161-1017

Endocrinology & Metabolic Syndrome
Open Access

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Research Article

Stress during Lactation Affects Fatty Acid Amide Hydrolase Protein Expression in Adipose Tissue and Liver of Adult Mice

Valeska AC, Carina AV, Paula P, Ana MR and Miguel NL*

Laboratory of Nutrition and Metabolic Regulation, Institute of Nutrition and Food Technology (INTA), University of Chile, Santiago, Chile

corresponding Author:
Miguel N. Llanos
Laboratory of Nutrition and Metabolic Regulation
Institute of Nutrition and Food Technology (INTA)
University of Chile, Santiago, El Líbano 5524, Santiago, Chile
Tel: +56-2-978-1507
Fax: +56-2-221- 4030;
E-mail: [email protected]

Received Date: October 24, 2014; Accepted Date: January 23, 2015; Published Date: February 03, 2015

Citation: Valeska AC, Carina AV, Paula P, Ana MR, Miguel NL (2015) Stress during Lactation Affects Fatty Acid Amide Hydrolase Protein Expression in Adipose Tissue and Liver of Adult Mice. Endocrinol Metab Synd 4:158. doi:10.4172/2161-1017.1000158

Copyright: © 2015 Valeska AC, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

 

Abstract

CIntroduction: Early stress alters the endo-cannabinoid system of tissues involved in energy homeostasis, leading to long term consequences associated to hormonal and metabolic disruptions associated to overweight/obesity and insulin resistance. We have previously reported a decreased Anandamide (AEA)-hydrolysing activity in liver and epididymal fat of adult mice previously subjected to stress during lactation, a result that may indicate reduced activity of the enzyme fatty acid amide hydrolase present in both tissues (FAAH; able to hydrolyse the endocannabinoid AEA). Objective: Since at present it is unknown whether early stress affects FAAH gene and/or protein expression in liver and adipose tissue during adulthood, we have performed studies with the aim of evaluating these parameters. Methods: Male mice pups were subjected to a soft nociceptive stress during the first 21 days of life (whole lactation period) with a subcutaneous injection of saline solution in the back, and sacrificed when adults (130 days old) to extract liver and epididymal fat. Results: Data obtained from real time PCR demonstrate that stress during lactation do not affect FAAH mRNA expression either in liver or epididymal adipose tissue of adult animals; however, Western Blot analyses indicates that FAAH protein amount was decreased by 35 and 65% in liver and epididymal fat, respectively. Conclusion: Present results demonstrate that nociceptive stress during lactation leads to decreased FAAH protein expression in liver and epididymal fat of adult mice, which may be associated to the low AEA- hydrolysing activity previously reported by us in both tissues. Low levels and activity of FAAH should result in increased availability of AEA, leading to a sustained activity of type 1 cannabinoid receptors associated to undesirable cellular and physiological consequences

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