alexa Structural Alterations in Scaffold Architecture in Response to Mechanical Stimuli
ISSN: 2157-7552

Journal of Tissue Science & Engineering
Open Access

Like us on:
OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article

Structural Alterations in Scaffold Architecture in Response to Mechanical Stimuli

Blackstone BN1,2, Wolever JD3 and Powell HM1,2,3*

1Department of Biomedical Engineering, The Ohio State University, Columbus, USA

2NSEC Center for Affordable Polymeric Biomedical Devices, The Ohio State University, Columbus, USA

3Department of Materials Science and Engineering, The Ohio State University, Columbus, USA

Corresponding Author:
Heather M. Powell
Department of Materials Science and Engineering
The Ohio State University, 243C Fontana Labs
Columbus, OH 43210, USA
Tel: 614-247-8673
Fax: 614-292-1537
E-mail: [email protected]

Received date: October 07, 2012; Accepted date: November 20, 2012; Published November 22, 2012

Citation: Blackstone BN, Wolever JD, Powell HM (2012) Structural Alterations in Scaffold Architecture in Response to Mechanical Stimuli. J Tissue Sci Eng 3:122. doi:10.4172/2157-7552.1000122

Copyright: © 2012 Blackstone BN, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

 

Abstract

Background: Though mechanical stimulation has been shown to improve mechanical properties of many engineered tissues, little is known about the impact of stimulation on scaffold architecture. In engineered tissues where the scaffold comprises a large portion of the tissue, mechanical signals are transferred from the external environment through the scaffold to the cells. Thus, a greater understanding of the architectural changes a scaffold experiences during mechanical stimulation may provide new knowledge on the communication between the cells and scaffold, during dynamic in vitro tissue development. Methods: Two distinct scaffold architectures were fabricated via lyophilization or electro-spinning of collagen. Pore size of lyophilized scaffolds, fiber diameter and inter-fiber distance of electrospun scaffolds, and ultimate tensile strength, linear stiffness and stress relaxation rates for all scaffolds were determined, prior to mechanical stimulation. Scaffolds were then subjected to 0, 5, 10 or 20% static or cyclic strain. Alignment of lyophilized scaffold pores and electrospun fibers was then quantified after 4 and 7 days of mechanical stimulation. Results: Lyophilized scaffolds displayed significant increases in pore area with magnitude of strain and duration of stimulation, and a pronounced alignment in pore orientation with the direction of strain. In contrast, electrospun
samples showed only modest changes in architecture, in response to applied mechanical strain with small (1-5%) increased in fiber alignment compared to control, and no observed changed in fiber morphology. Conclusion: The current study showed the initial degree of interconnectivity between scaffold elements greatly impacted the scaffold response to mechanical stimulation. Non-woven electro-spun scaffold undergo very little micro structural reorganization during mechanical stimulation, while lyophilized collagen scaffolds undergo significant micro-structural changes. These differences in scaffold response suggest that large stain magnitudes may be required to excite cells within electro spun scaffolds, while modest strain magnitudes may results in significant changes in cellular behavior within lyophilized collagen sponges.

Share This Page

Additional Info

Loading
Loading Please wait..
 
Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri, Food, Aqua and Veterinary Science Journals

Dr. Krish

[email protected]

1-702-714-7001 Extn: 9040

Clinical and Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemical Engineering and Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001 Extn: 9040

Earth & Environmental Sciences

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

General Science and Health care Journals

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics and Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001 Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Informatics Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Material Sciences Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Mathematics and Physics Journals

Jim Willison

[email protected]

1-702-714-7001 Extn: 9042

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001 Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

John Behannon

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001 Extn: 9042

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords