alexa STUDIES OF CYPERMETHRIN ACTIVITY ON STEROIDOGENESIS IN


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Research Article

STUDIES OF CYPERMETHRIN ACTIVITY ON STEROIDOGENESIS IN FEMALE LABEO ROHITA

Sanjeeva Prasad Tarigopula1* and Philip G.H1
  1. Department of Zoology. Sri Krishnadevaraya University, Anantapuramu, A.P, INDIA
Corresponding Author: Sanjeeva Prasad Tarigopula, E-mail: [email protected]
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Abstract

A growing number of environmental toxicants like synthetic pyrethroids are believed to have deleterious effects on the development of non-target organisms by disrupting hormone sensitive processes. LC50 values of different forms of cypermethrin were evaluated by different research groups under laboratory conditions. In this work, the plasma concentrations of Vitellogenin (VTG), 17β-estradiol (E2), testosterone (T), 17, 20β-dihydroxy-4- pregnen-3-one and Aromatase were evaluated in female Labeo Rohita. Labeo rohita were purchased from the local fisheries and fed with fish feed. Six female fish were kept in 500L water tanks and then exposed to 10 and 20 μg/L of Cypermethrin for six days. Vitellogenin, 11-Keto testosterone, Testosterone, 20β-dihydroxy-4-pregnen-3-one and Aromatase were estimated using kits from Biosense laboratories by Enzyme linked immune sorbent assay (ELISA). In female fish, testosterone levels in female have increased with increase in concentration of the toxicant and decrease of 17β-estradiol in exposed fish with increase in cypermethrin concentration. The levels of Vitellogenin and Aromatase have no significant change with increase of Cypermethrin.

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