Study DNA Damage after Photodynamic Therapy using Silver Nanoparticles with A549 cell lineEl-Hussein A*
The National Institute of Laser Enhanced Science, Cairo University, Egypt
- *Corresponding Author:
- Ahmed El-Hussein
The National Institute of Laser Enhanced Science
Cairo University, Egypt
Tel: 20 2 35715882
E-mail: [email protected]
Received Date: December 23, 2015 Accepted Date: Janaury 11, 2016 Published Date: January 20, 2016
Citation: El-Hussein A (2016) Study DNA Damage after Photodynamic Therapy using Silver Nanoparticles with A549 cell line. J Nanomed Nanotechnol 7:346. doi:10.4172/2157-7439.1000346
Copyright: & copy; 2016 El-Hussein A. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Lung Cancer is considered one of the major health problems worldwide. Nanomedicine is considered one of the promising research applications nowadays. This is due to the unique physical and chemical properties of the nanoscale elements. Silver nanoparticles (Ag NPs) in specific has been extensively studied recently in many biomedical applications especially in cancers, since they possess multifunctional effects that make these nanostructures ideal candidates for biomedical applications. Ag NPs were proved to have anti- tumor activity with apoptotic cell death pathway. The goal of the current work was to investigate the degree of DNA damage that results from the usage of Ag NPs as a photosensitizer (PS) in photodynamic therapy (PDT), besides to evaluate the reactive oxygen species (ROS) yield that was associated. The results showed the occurrence of DNA damage in lung cancer cells (A549) through the generation of ROS via the detection of the mitochondrial membrane potential changes.