alexa Study of Interaction Between AviPro<sup>andreg;</sup> <em>Mycoplasma gallisepticum</em> (MGF) and Pulmotil<sup>andreg;</sup>-AC in Broiler Breeder Pullets| Abstract
ISSN: 2157-7579

Journal of Veterinary Science & Technology
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  • Research Article   
  • J Vet Sci Technol 10: 585,

Study of Interaction Between AviPro® Mycoplasma gallisepticum (MGF) and Pulmotil®-AC in Broiler Breeder Pullets

Mohamad T Farran*, Majd G Kais, Nour M Ramadan, Houssam A Shaib, Mohammad J Kaddoura and Omar S Jaroush
Faculty of Agriculture and Food Sciences, Department of Agriculture, American University of Beirut, Beirut, Lebanon
*Corresponding Author : Dr. Mohamad T Farran, Faculty of Agricultural and Food Sciences, Department of Agriculture, American University of Beirut, Riad El Solh 1107- 2020, Beirut, Lebanon, Tel: +9611350000, Fax: +961744460, Email: [email protected]

Received Date: Jul 22, 2019 / Accepted Date: Jul 31, 2019 / Published Date: Aug 07, 2019

Abstract

This study aimed at the determination of the optimal time interval between the vaccination with a live Mycoplasma gallisepticum vaccine AviPro® MGF and the administration of the antibiotic Pulmotil® AC (PAC) to broiler breeder pullets while preserving the vaccine efficacy. A total of 108 sixteen-weeks-old breeder pullets of the ROSS 308 strain were subdivided equally into 6 groups. Pullets of group 1 remained unvaccinated and untreated with PAC. Birds of groups 3, 4, 5 and 6 were vaccinated with AviPro® MGF at 16 weeks of age and treated with PAC at 3, 7, 14, and 21 days post-vaccination respectively. Group 2 was kept as the vaccinated non-treated group. The pullets were tested for the presence of MGF strain in the trachea at different days after PAC-treatment completion. All PAC-treated groups showed tracheal MG recolonization after the treatment was discontinued. The percentage of positive tracheal MG swab cultures was consistently higher in group 6, reaching a plateau at 14 days post PAC treatment (100%, P<0.05). qPCR implied-tracheal MG counts indicated better recolonization efficiency for birds of groups 5 and 6 reaching up to 2322 × 103 and 2839 × 103 cfu/ml of broth, respectively, at 35 days post PAC treatment. Moreover, Group 6 showed the significantly highest titer to MG, recording a value of 2160, followed by the vaccinated untreated Group 2 (1128). For a successful application of live MG vaccine/antibiotics combination, it is recommended to delay PAC treatment 21 days after the vaccination of breeder pullets with AviPro® MGF.

Keywords: Mycoplasma gallisepticum; AviPro MGF; Pulmotil AC; Pullets; qPCR; ELISA

Citation: Farran MT, Kais MG, Ramadan NM, Shaib HA, Kaddoura MJ, et al. (2019) Study of Interaction Between AviPro® Mycoplasma gallisepticum (MGF) and Pulmotil®-AC in Broiler Breeder Pullets. J Vet Sci Technol 10: 585.

Copyright: © 2019 Farran MT, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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