Study of Local Anaesthetics. Part 203*. Micellization of Heptacainium Chloride Studied By Spectral Methods in Aqueous Solution
- *Corresponding Author:
- Jana Galisinova
Comenius University in Bratislava
Faculty of Pharmacy
Department of Pharmaceutical Chemistry
Odbojárov 10, 832 32 Bratislava
E-mail: [email protected]
Received Date: April 27, 2013; Accepted Date: May 17, 2013; Published Date: May 24, 2013
Citation: Gališinová J, Andriamainty F, Cižmárik J, Malík I, Sichrovská L, et al. (2013) Study of Local Anaesthetics. Part 203*. Micellization of Heptacainium Chloride Studied By Spectral Methods in Aqueous Solution. J Bioequiv Availab 5:161-164. doi: 10.4172/jbb.1000152
Copyright: © 2013 Gališinová J, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
The formation of micelles of the local anaesthetic heptacainium chloride (substance, XIX) in aqueous solution was investigated by the pyrene absorption and the pyrene I 1 /I 3 ratio methods, respectively by optical density (OD) method for surface curvature measurement. First, the absorption spectroscopy in UV/VIS region was based on studying changes in characteristic absorption spectrum of pyrene in presence of surfactant. The resultant plot of the sum of absorbances for the entire key pyrene maximum as a function of the total surfactant concentration showed, in the region of the critical micellar concentration, a usual sigmoidal increase. The fluorescence emission spectroscopy in UV/VIS region of spectrum by the probe pyrene, second procedure, was applied for determination of the cmc from the detections of the pyrene I 1 /I 3 ratio as a function of the surfactant concentration. The pyrene ratio data were fitted by the Boltzmann-type sigmoid of declining progress. The third (OD) method used required microliters of surfactant solutions and standard microplate absorbance measurements.