The present study deals with comparative study of the periodic and aperiodic variations of Physicochemical status of two lakes, Nardipur and mansa of Gandhinagar District, Gujarat India. Nardipur Lakes was situated on Mansa-Kalol highway of Gujarat state and mansa lake was situated in village of mansa. Both Lakes having natural freshwater body and irregular in shape. Nardipur lake is located between 23020’09’’N to 23020’11’’N longitude and 72033’47’’E to 72034’03’’E Latitude and mansa lake is located between 23025’47’’N to 23025’49’’N longitude and 72039’16’’E to 72039’26’’E Latitude. Both are the oldest lake. Water is essential for living organisms. A comparative study of the periodic and aperiodic variation of Physico-chemical status of two lakes was studied in January to June months of year 2011. Both the lakes are biologically affected by various anthropogenic activities. In the present study water characteristics of two lakes have been compared as water quality. Different Parameters were analyzed like pH, Fluoride, COD, BOD, Chloride, Alkalinity, Total Hardness, Calcium, Calcium Hardness, Magnesium, Magnesium Hardness, DO, EC and TDS. The result indicates that the both lakes are in polluted condition.