Study of Tissue Printing Parameters for Generating Complex Tissue ConstructsG Navarro1*, I Garcia1, PA Sundaram1 and N Diffoot-Carlo2
- *Corresponding Author:
- Geoffrey Navarro Vale
B.S Recinto Universitario de Mayaguez Universidad de Puerto Rico
Isabela, PR Puerto Rico
E-mail: [email protected]
Received date: July 22, 2016; Accepted date: August 12, 2016; Published date: August 20, 2016
Citation: Navarro G, Garcia I , Sundaram PA, Carlo ND (2016) Study of Tissue Printing Parameters for Generating Complex Tissue Constructs. J Tissue Sci Eng 7:175. doi:10.4172/2157-7552.1000175
Copyright: © 2016 Navarro G, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
A mixture of agarose, MEM IX and HeLa cells (dubbed Bio-Ink) was created to allow normal cell interaction with the scaffold material (agarose) before crosslinking as an initial step in 3D printing tissue. Bio-Ink was developed successfully as an in situ-scaffolding material for engineering biological structures. Bio-Ink has been further conditioned by adjusting agarose composition and gelling time to obtain optimal HeLa cell growth. After detailed study, the time range available for printing this material, before full crosslinking occurs, was determined to be about 300 s, giving it attractive properties for 3D printing. Repeatable 10 mm thick prints were successful, although more system calibration is still needed to achieve more complex prints.