alexa Study on Interactions between Fed-Batch and Batch Operating Parameters for the Development of Monoclonal Antibody Fed-Batch using Design of Experiments | Abstract
ISSN: 2155-9821

Journal of Bioprocessing & Biotechniques
Open Access

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Research Article

Study on Interactions between Fed-Batch and Batch Operating Parameters for the Development of Monoclonal Antibody Fed-Batch using Design of Experiments

Junaid Muneer Raja1,2, Nurina Anuar1*, Badarulhisam Abdul Rahman2 and Jamaliah Md Jahim1
1Department of Chemical and Process Engineering, Faculty of Engineering and Built Environment, University Kebangsaan Malaysia, 43600 UKM, Bangi, Selangor, Malaysia
2InnoBiologics Sdn. Bhd., Lot 1, Persiaran Negeri, 71800 Nilai, Negeri Sembilan, Malaysia
Corresponding Author : Nurina Anuar
Department of Chemical and Process Engineering
Faculty of Engineering and Built Environment
University Kebangsaan Malaysia
43600 UKM, Bangi, Selangor, Malaysia
Tel: 603-89213132
E-mail: [email protected]
Received May 29, 2014; Accepted June 28, 2014; Published July 06, 2014
Citation: Raja JM, Anuar N, Rahman BA, Jahim JM (2014) Study on Interactions between Fed-Batch and Batch Operating Parameters for the Development of Monoclonal Antibody Fed-Batch using Design of Experiments. J Bioprocess Biotech 4:165 doi: 10.4172/2155-9821.1000165
Copyright: © 2014 Raja JM, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

In conventional fed batch process development approaches, batch operating parameters (such as pH, temperature, seeding density, dissolved oxygen concentration) are kept constant and only feeding parameters such as feeding time, post-feed concentration are manipulated. The batch and fed batch operating parameters are assumed to be independent of each other. This approach to process development ignores any interactions that might exist between the batch and fed-batch operating parameters are therefore not evaluated. However in a complex bioprocess, none of the factors affecting the process can be assumed to be independent of each other and mutually exclusive. Therefore in this study an attempt was made to study the interaction between fed-batch operating parameter-post feed glucose concentration (A) and batch operating parameters- seeding density (B), temperature (C), and dissolved oxygen concentration (D) by their simultaneous manipulation, as well as the effect of these interactions on cell growth and monoclonal antibody (mAb) production. NS0 cell line producing the mAb’s against carcino-embryogenic antigen (Anti-CEA) was used. The final mAb concentration, viable cell density and integral of viable cell concentration (IVCC) were the responses evaluated. Statistical analysis experimental data showed that parameter (A) and its interaction with parameter (B) were the main factors affecting both the response variables. In comparison to the batch run which yielded 5.21 mg/L mAb, the developed fed batch process increased the mAb titer by 10 fold (59.40 mg/mL), and the IVCC was increased by 7 fold. The maximum VCD value (3.46×106 cells/mL) of the developed fed batch process was 1.25 over fold the value for batch.

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