alexa Study on Isolation, Optimization and Immobilization of Alkaline Protease produced by Aspergillus Flavus
ISSN: 2322-0066

Research & Reviews: Research Journal of Biology
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Research Article

Study on Isolation, Optimization and Immobilization of Alkaline Protease produced by Aspergillus Flavus

Roshni Choubey1*,Ram Keerti Verma2, Ravi Prakash Mishra3 and Pooja Sharma4

1Department of Botany and Microbiology, St. Aloysius’ College (Auto.), Jabalpur, India

2Forest Pathology Division, Tropical Forest Research Institute, Jabalpur, India

3Department of Post Graduate Studies and Research in Biolo gical Sciences, Rani Du rgavati Vishwavidyalaya, Jabalpur, India

4Department of Botany and Microbiology, St. Aloysius’ College (Auto.), Jabalpur, India

Corresponding Author:
Roshni Choubey
Department of Botany and Microbiology, St. Aloysius’ College (Auto.)
Jabalpur, India
E-mail: [email protected]

Received Date: 14/12/2016; Accepted Date: 13/06/2016; Published Date: 15/06/2016



This study was conducted to isolate alkaline protease producing fungus, its identification and optimization of cultural conditions for production of alkaline protease enzyme. Preliminary screening was done on gelatin agar medium. Secondly gelatin liquification test was carried out to check the production of proteases functional at alkaline pH. The study related to process development involves optimization of different fermentation conditions towards enhancement of enzyme production for which the cultural conditions (physical and nutritional factors) during solid state fermentation for alkaline protease production by the isolated Aspergillus flavus were undertaken. Different types of substrates were selected for growing Aspergillus flavus. Alkaline protease production was found to be highest at temperature (280C), pH 8, incubation time 168 hrs, with metal ions (Mn), nitrogen source (peptone), and carbon source (sucrose) as the biggest zone of hydrolysis. The highest optical density and protein concentration was obtained at above mentioned conditions. The partial purification of alkaline protease enzyme from the culture filtrate was performed by ammonium sulfate salt precipitation which was immobilized using sodium alginate immobilization technique. Washing test resulted in complete removal of blood stain which indicates this enzyme can be proved crucial for detergent industry.


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