alexa Study on the Effect of pH, Temperature and Aeration on the Cellular Growth and Xanthan Production by Xanthomonas campestris Using Waste Residual Molasses
ISSN: 2155-9821

Journal of Bioprocessing & Biotechniques
Open Access

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Research Article

Study on the Effect of pH, Temperature and Aeration on the Cellular Growth and Xanthan Production by Xanthomonas campestris Using Waste Residual Molasses

P. Mudoi*, P. Bharali and B. K. Konwar
Department of Molecular Biology and Biotechnology, Tezpur University, Tezpur, Assam-784028, India
Corresponding Author : Mudoi P
Department of Molecular Biology and Biotechnology
Tezpur University, Tezpur
Assam-784028, India
Tel: +91-9435442667
Email: [email protected]
Received July 02, 2013; Accepted September 27, 2013; Published October 08, 2013
Citation: Mudoi P, Bharali P, Konwar BK (2013) Study on the Effect of pH, Temperature and Aeration on the Cellular Growth and Xanthan Production by Xanthomonas campestris Using Waste Residual Molasses. J Bioprocess Biotech 3:135 doi: 10.4172/2155-9821.1000135
Copyright: © 2013 Mudoi P, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

Waste residual molasses, a non-edible portion produced during the processing of sugarcane juice for the preparation of molasses, may be an alternative low-cost renewable substrate to the pricey food-grade molasses for xanthan production. Systematic strategies were applied to improve xanthan production with a newly isolated indigenous strain Xanthomonas campestris originated from Tezpur, Assam. Analyses with TLC, HPLC and FTIR show that the polymer consisted mainly of glucose, galactose and glucornic acid but showed no evidence of xylose, arabinose or glycoprotein in the polysaccharide. The isolated xanthan exhibited all the required physico-chemical characteristics and were examined by using TGA, DSC, XRD and SEM. Maximum concentration of xanthan was observed after 24h of incubation of the culture media, pH 7 at 28°C with 200 rpm. The viscosity of xanthan was found to be stable over a wide range of pH, reduced with the increase in temperature and raised at the higher xanthan concentration. The results obtained in the present investigation are noteworthy for the possible xanthan production from low-cost waste residual molasses at an industrial level.

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