Study on the Prevalence and Distribution of Staphylococcus aureus in Raw Cow Milk Originated from Alage Atvet College Dairy Farm, EthiopiaDerebe Tessema1 and Solomon Tsegaye2*
- *Corresponding Author:
- Solomon Tsegaye
College of Agriculture
Woldia University, Woldia, Ethiopia
Tel: +251 913 733 048
E-mail: [email protected]
Received Date: March 18, 2017; Accepted Date: March 20, 2017; Published Date: March 28, 2017
Citation: Tessema D, Tsegaye S (2017) Study on the Prevalence and Distribution of Staphylococcus aureus in Raw Cow Milk Originated from Alage Atvet College Dairy Farm, Ethiopia. J Nutr Food Sci 7:586. doi: 10.4172/2155-9600.1000586
Copyright: © 2017 Tessema D, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
This investigation was carried out from October 2011-June 2012 to determine the prevalence of Staphylococcus in milk and rate of contamination at farm and milk distribution tank in Alage Agricultural Technical Vocational Educational Training College. Milk samples (row) were cultured on sheep blood agar and incubated. The plates were examined for gross colony morphology, pigmentation and haemolytic characteristics at 24-48 h then presumptive colonies of Staphylococcus aureus was selected and sub cultured on nutrient agar and incubated. Then, bacteria were identified according to its Gram reaction, morphology and the catalase test, tube coagulase test (4 h), haemolysis, pigment production (golden yellow), mannitol and maltose fermentation were used. A total of 170 milk sample of cow were examined for bacteriological status of S. aureus, an overall 28.2% prevalence of Staphylococcus was found. From this, 21.2% was directly from the farm and 35.3% were from milk distributing site to consumers. The total prevalence of Staphylococcus varied among the sample taking site. The prevalence of S. aureus from distributing raw milk site (35.3%) was significantly higher than the prevalence of S. aureus from samples taken directly from the farm (21.2%). From this the study it is considered that Staphylococcosis a potential hazard for the public and contamination rate is high in distribution site which needs improvement of the hygienic status of the personnel’s at distribution site.