Study on the Relationship between the Structure and Functions of Antihuman Cervical Cancer Single-chain Antibody and the Lengths of Linkers
- *Corresponding Author:
- Quxiao Du
People’s Hospital of Zhengzhou
No. 33 Huanghe Road
Zhengzhou, 450003, China
E-mail: [email protected]
Received date: November 05, 2014; Accepted date: December 24, 2014; Published date: December 29, 2014
Citation: Cheng J, Du Q, Zhang X, Ren Y, Zhang B, Feng X (2014) Study on the Relationship between the Structure and Functions of Anti-human Cervical Cancer Single-chain Antibody and the Lengths of Linkers. J Proteomics Bioinform S8:004. doi: 10.4172/jpb.S8-004
Copyright: © 2014 Cheng J, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Background: This study aimed to find the linker length with minimal impact among chains to fight against the structure and function of cervical cancer single-chain antibody.
Methods: The original variable region of heavy chain (VH) and variable region of light chain (VL), and the singlechain antibody with (Gly4Ser)n linkers of different lengths (n=1~8) were modeled using SWISS-MODEL homologous modeling. Comparison of the similarity of original VH/VL and VHn/VLn was carried out by applying the algorithm of spatial hierarchical alignment based on the spherical coordinates. The fore and aft distance and diffusion radius of α were also calculated. The stability of antibody with different linker length was then compared. ELISA method was adopted to evaluate the immunological activity of single-chain antibody with different linkers. MTT assay was used to analyze the inhibition effect of ScFv-n on cervical cancer cells.
Results: When linker unit n=4, the structures were the most similar between ScFv and the original VH/VL. When n=3, the similarity of it had little differences to that when n=4, and the influence of adding connecting peptide on the stability of single-chain antibody was the least, when n=3. The result of ELISA and MTT methods indicated that when n=3, single-chain antibody gained the highest activity.
Conclusion: The optimum length of linker of anti-human cervical cancer single-chain antibody was n=3 from the point of mathematical modeling and biology experiments. This study provided new ideas for the design and constructions of single-chain antibody, and theoretical basis for the treatment of cervical cancer.