alexa Sugarcane Cystatin CaneCPI-4 inhibits Melanoma Growth by Angiogenesis Disruption
ISSN: 1948-5956

Journal of Cancer Science & Therapy
Open Access

Like us on:
OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article

Sugarcane Cystatin CaneCPI-4 inhibits Melanoma Growth by Angiogenesis Disruption

Juliana P Oliveira1, Helena F Magliarelli1, Felipe V Pereira2, Andréia Gianotti3, Andréa Soares-Costa3, Flavio Henrique-Silva3, Alda Wakamatsu4, Iberê C Soares4, Suely Nonogaki5, Luiz R Travassos2, Adriana K Carmona1 and Thaysa Paschoalin2*

1Departamento de Biofísica, Universidade Federal de São Paulo, São Paulo, Brazil

2Departamento de Microbiologia, Imunologia e Parasitologia, Unidade de Oncologia Experimental (UNONEX), Universidade Federal de São Paulo, São Paulo, Brazil

3Laboratório de Biologia Molecular, DGE, Universidade Federal de São Carlos, São Paulo, Brazil

4LIM14 – Faculdade de Medicina, Universidade de São Paulo, São Paulo, Brazil

5Instituto Adolfo Lutz, São Paulo, Brazil

*Corresponding Author:
Dr. Thaysa Paschoalin
Unidade de Oncologia Experimental
Departamento de Microbiologia
Imunologia e Parasitologia
Universidade Federal de São Paulo (UNIFESP)
Rua Botucatu, 862, 8º andar
São Paulo, SP 04023-062, Brazil
Tel: +55-11-5576-4551
Fax: +55-11-5571-5877
E-mail: [email protected]

Received Date: August 09, 2011; Accepted Date: September 07, 2011; Published Date: September 15, 2011

Citation: Oliveira JP, Magliarelli HF, Pereira FV, Gianotti A, Soares-Costa A, et al. (2011) Sugarcane cystatin CaneCPI-4 inhibits Melanoma Growth by Angiogenesis Disruption. J Cancer Sci Ther 3: 161-167. doi:10.4172/1948-5956.1000081

Copyright: © 2011 Oliveira JP, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.



Study background: Cathepsins are lysosomal cysteine proteases that have increased expression in tumor cells, may translocate to the cell surface and be secreted. They play a role in tumor angiogenesis. Cystatins are natural cysteine protease inhibitors that can inhibit tumor development, growth and metastasis. In the present work we evaluated the potential therapeutic use of sugarcane cystatin CaneCPI-4 as an anticancer drug. Methods: Viability, migration, invasion and anchorage-independent growth were investigated in B16F10-Nex2 melanoma and HUVEC cells in the presence of CaneCPI-4. The in vivo effect of CanceCPI-4 on tumor development was assessed using a murine model. Angiogenesis in vitro was evaluated using HUVEC cells plated on Matrigel. Immunohistochemical analysis of CD34 expression in primary melanoma was also carried out. Results: Sugarcane cystatin CaneCPI-4 was not cytotoxic to melanoma or HUVEC cells growing in vitro, but efficiently inhibited melanoma cell development in vivo. CaneCPI-4 inhibited melanoma and endothelial cell migration and tumor invasion in vitro. Using a Matrigel angiogenesis assay, CaneCPI-4 at 1 mM was able to completely abolished endothelial cell sprouting in vitro. Angiogenesis inhibition was confirmed in vivo by immunohistochemistry. Conclusions: Sugarcane cystatin CaneCPI-4 inhibits melanoma development in vivo by angiogenesis disruption and inhibition of melanoma invasion, migration and anchorage-independent growth.


Share This Page

Additional Info

Loading Please wait..
Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri, Food, Aqua and Veterinary Science Journals

Dr. Krish

[email protected]

1-702-714-7001 Extn: 9040

Clinical and Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals


[email protected]

1-702-714-7001Extn: 9042

Chemical Engineering and Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001 Extn: 9040

Earth & Environmental Sciences

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

General Science and Health care Journals

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics and Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001 Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Informatics Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Material Sciences Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Mathematics and Physics Journals

Jim Willison

[email protected]

1-702-714-7001 Extn: 9042

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001 Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

John Behannon

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001 Extn: 9042

© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version