alexa Sulfadoxine-Pyrimethamine Resistant Haplotypes in Asymptomatically and Symptomatically Malaria Infected Individuals in Côte d’Ivoire
ISSN: 2470-6965

Malaria Control & Elimination
Open Access

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article

Sulfadoxine-Pyrimethamine Resistant Haplotypes in Asymptomatically and Symptomatically Malaria Infected Individuals in Côte d’Ivoire

Berenger Aristide Ako Ako1,2*, Marnie Johansson2, Rokia Traore1, Toure André Offianan1, Eric Adji Gbessi1, Coulibaly Mangoa Yahya3, Simon-Pierre Assanvo Nguetta3, Louis Koné Penali1 and Carol Hopkins Sibley2

1Department of Malariology, Institut Pasteur de Côte d'Ivoire, Abidjan, Côte d'Ivoire

2Department of Genome Sciences, University of Washington, Seattle, WA, USA

3Laboratoire de Génétique, UFR Biosciences, Université Félix Houphouët-Boigny, Abidjan, Côte d'Ivoire

*Corresponding Author:
Berenger Aristide Ako Ako
Malariology Department of
Institut Pasteur de Côte d’Ivoire BP 490 Abidjan
Côte d'Ivoire
Tel: +225 05 63 67
Email: ako.aab@gmail.com

Received Date: December 26, 2013; Accepted Date: March 06, 2014; Published Date: March 12, 2014

Citation: Berenger Aristide Ako Ako, Marnie Johansson, Rokia Traore, Toure André Offianan, Eric Adji Gbessi, et al. (2014) Sulfadoxine-Pyrimethamine Resistant Haplotypes in Asymptomatically and Symptomatically Malaria Infected Individuals in Côte d’Ivoire. Malar Chemoth Cont Elimination 3:112. doi:10.4172/ 2090-2778.1000112

Copyright: © 2014 Berenger AAA, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

 

Abstract

Background: Most studies that assess efficacy of antimalarial drugs focus on the outcome of clinical treatment. However, community surveys of surrogate indicators are often more practical and can provide a wider view of possible changes in drug response, but it has not been clear whether assessment of parasite isolates from patients and asymptomatic individuals are directly comparable. In the present work, we have compared the prevalence of molecular markers associated with resistance to sulfadoxine-pyrimethamine in parasites isolated from asymptomatic and symptomatic individuals. Methods: The study was conducted during April and May 2008 in Anonkoua-Kouté (Abobo-Abidjan) in southern Côte d’Ivoire, an area where SP has been intensively used for more than 20 years. Plasmodium falciparum monospecific infection was detected by blood smears, followed by a genomic DNA extraction from blood spots on filter paper. Extracted DNA was amplified by nested-PCR, and pfdhfr and pfdhps sequences analyzed. Results: Ninety six of 107 asymptomatic schoolchildren sampled were positive for P. falciparum; 48 of these isolates were used for molecular analysis. A subset of 67 samples from malaria patients of school age was analyzed in parallel. For pfdhfr, the wild-type NCSI and the triple mutant IRNI alleles were both present in about 30 and 50% of the isolates from asymptomatic children and symptomatic malaria patients, respectively. For pfdhps, the symptomatic children mostly carried the single mutant genotype SGKAA although the double mutant AGKAA was the predominant allele in both populations. Conclusions: Direct comparison of molecular markers of SP resistance demonstrates that the prevalence of these alleles is comparable in isolates derived from asymptomatic and symptomatic individuals. The results from this study support the possibility of using cross sectional surveys of surrogate molecular markers of SP efficacy to inform decisions about choice of drugs for intermittent preventive treatment of pregnant women or seasonal malaria chemoprophylaxis.

Keywords

Share This Page

Additional Info

Loading
Loading Please wait..
 
Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri, Food, Aqua and Veterinary Science Journals

Dr. Krish

agrifoodaquavet@omicsonline.com

1-702-714-7001 Extn: 9040

Clinical and Biochemistry Journals

Datta A

clinical_biochem@omicsonline.com

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

business@omicsonline.com

1-702-714-7001Extn: 9042

Chemical Engineering and Chemistry Journals

Gabriel Shaw

chemicaleng_chemistry@omicsonline.com

1-702-714-7001 Extn: 9040

Earth & Environmental Sciences

Katie Wilson

environmentalsci@omicsonline.com

1-702-714-7001Extn: 9042

Engineering Journals

James Franklin

engineering@omicsonline.com

1-702-714-7001Extn: 9042

General Science and Health care Journals

Andrea Jason

generalsci_healthcare@omicsonline.com

1-702-714-7001Extn: 9043

Genetics and Molecular Biology Journals

Anna Melissa

genetics_molbio@omicsonline.com

1-702-714-7001 Extn: 9006

Immunology & Microbiology Journals

David Gorantl

immuno_microbio@omicsonline.com

1-702-714-7001Extn: 9014

Informatics Journals

Stephanie Skinner

omics@omicsonline.com

1-702-714-7001Extn: 9039

Material Sciences Journals

Rachle Green

materialsci@omicsonline.com

1-702-714-7001Extn: 9039

Mathematics and Physics Journals

Jim Willison

mathematics_physics@omicsonline.com

1-702-714-7001 Extn: 9042

Medical Journals

Nimmi Anna

medical@omicsonline.com

1-702-714-7001 Extn: 9038

Neuroscience & Psychology Journals

Nathan T

neuro_psychology@omicsonline.com

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

John Behannon

pharma@omicsonline.com

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

social_politicalsci@omicsonline.com

1-702-714-7001 Extn: 9042

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords