Supplementation Diet Containing Probiotics, Herbal And Azadirachtin On Hematological And Biochemical Changes In Cirrhina mrigala Against Aphanomyces invadansRamasamy Harikrishnan1*, Chellam Balasundaram2, MoonSoo Heo1
1Marine Applied Microbes & Aquatic Organism Disease Control Lab, Department of Aquatic Biomedical Sciences, School of Marine Biomedical Sciences, Marine and Environmental Research Institute, College of Ocean Science, Jeju National University, Jeju 690756, South Korea
- *Corresponding Author:
- Ramasamy Harikrishnan
E-mail: [email protected]
Published date: May 24, 2010
Cirrhina mrigala (63 ± 2 g) were intramuscularly (i.m.) administered with 2.3 x 107 CFU ml-1 of Aphanomyces invadans (isolate B99C). The hematological and biochemical parameters were studied in the experimental and control groups for 30 days. In infected untreated group (I), the white blood cell count (WBC: 106mm-3) was significantly increased (P < 0.05) from the control, while no change was seen in groups fed probiotics, herbal and azadirachtin supplementation diets. Similar trend was noted in the haemoglobin (Hb: g/dL) and haematocrit (Hct: %) levels. Interestingly, infected fish fed probiotics, herbal and azadirachtin supplementation diets, did not differ (P > 0.05) from the control. A similar trend prevailed in the percentage of lymphocytes (LYM), monocytes (MON), eosinophils (EOS), and neutrophils (NEU). The total protein (TP: g dl-1), glucose (GLU: mg dl-1), calcium (CAL: mmol l-1), and cholesterol (CHO mmol l-1) levels were affected (P < 0.05) in the infected group. The present study suggests that the administration of probiotics, herbal, and azadirachtin supplementation diets for 30 days protects the hematological and biochemical parameters in C. mrigala from A. invadans.