Surface Modification of Superparamagnetic Magnetite Nanoparticles and Its Application for Detection of Anti-CEA Using Electrochemiluminescent Immunosensor
Guixin Huang, Biyang Deng*, Qing Xi, Chunyao Tao and Li Ye
Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources (Ministry of Education of China), School of Chemistry and Pharmaceutical Sciences, Guangxi Normal University, Guilin 541004, China
- *Corresponding Author:
- Biyang Deng
Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources
(Ministry of Education of China)
School of Chemistry and Pharmaceutical Sciences
Guangxi Normal University, Guilin 541004, China
E-mail: [email protected]
Received date: December 17, 2014; Accepted date: January 29, 2015; Published date: January 31, 2015
Citation: Huang G, Deng B, Xi Q, Tao C, Ye L (2015) Surface Modification of Superparamagnetic Magnetite Nanoparticles and Its Application for Detection of Anti-CEA Using Electrochemiluminescent Immunosensor. Med chem 5:050-057. doi:10.4172/2161-0444.1000242
Copyright: © 2015 Huang G, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
A highly sensitive sandwich-type ECLIA technique using ruthenium complexes as labels was reported. Superparamagnetic nanoparticles were synthesized with a diameter of 12 nm through a coprecipitation method and used as the matrix solid-phase. Then, the ECL targets of Ru(bpy)2(dcbpy)NHS were successfully synthesized and labeled on the IgG. Furthermore, on the formation of an antibody-antigen immunocomplex, biotin-streptavidin system and on the basis of the strong and stable ECL signal, we designed a novel multiple signal amplification of sandwichtype ECLIA for sensitive detection of carcinoembryonic antigen (CEA) antibody. The calibration curve was linear in the range of 1.0–780 ng mL–1 with a detection limit of 0.28 ng mL–1 (σ = 3) and relative standard deviation of 3.9% (n = 6). The results indicated that the new sandwich-type ECLIA provide several advantages, such as high sensitivity, good selectivity, and wide linear range. This technique can be used in detection of anti-CEA as well as of other tumor markers.