alexa Surface Regeneration of Gold-Coated Chip for Highly-Reproducible Surface Plasmon Resonance Immunoassays
ISSN: 2155-6210

Journal of Biosensors & Bioelectronics
Open Access

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Research Article

Surface Regeneration of Gold-Coated Chip for Highly-Reproducible Surface Plasmon Resonance Immunoassays

Chandra Kumar Dixit1,2,3*

1 Department of Mechanical Engineering, Technion-Israel Institute of Technology, Israel

2 Centre for Bioanalytical Sciences (CBAS), National Centre for Sensor Research, Dublin City University, Dublin 9, Ireland

3 Applied Biochemistry Group, School of Biotechnology, Dublin City University, Dublin 9, Ireland

*Corresponding Author:
Chandra Kumar Dixit
Department of Mechanical Engineering
Technion-Israel Institute of Technology, Israel
Tel: +972546447363
E-mail: [email protected]

Received Date: March 26, 2014; Accepted Date: April 28, 2014; Published Date: May 05, 2014

Citation: Dixit CK (2014) Surface Regeneration of Gold-Coated Chip for Highly- Reproducible Surface Plasmon Resonance Immunoassays. J Biosens Bioelectron 5:149. doi: 10.4172/2155-6210.1000149

Copyright: © 2014 Dixit CK. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

We developed a simple procedure for the complete regeneration of silane-grafted gold surface. This method allowed the reuse of the regenerated gold chip for custom refunctionalization. In addition, we performed highly reproducible immunoassays using these chips over several cycles. The developed procedure was optimized and comprised of consecutive treatments of functionalized Au chip with 12 M HCl for 10 min and 29 W oxygen (O2)-plasma for 5 min. Monitoring and surface characterization of the developed methodology was performed with ellipsometry and Rutherford back scattering. The developed procedure was demonstrated on SPR-based Human Fetuin A (HFA) immunoassay, where the amino groups of APTES-functionalized Au chip were cross linked to the carboxyl groups of anti-HFA antibody using 1-ethyl-3-[3-dimethylaminopropyl] carbodiimide hydrochloride and sulfo-N-hydroxy succinimide. The APTES-functionalization, anti-HFA antibody immobilization and HFA binding on the regenerated SPR Au chip were highly reproducible over 40 HFA immunoassay cycles.

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