alexa Surveillance on Avian Influenza H5N1 and H9N2 Subtypes
ISSN: 2375-446X

Poultry, Fisheries & Wildlife Sciences
Open Access

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Research Article

Surveillance on Avian Influenza H5N1 and H9N2 Subtypes In Egypt 2012-2013

Shakal MA1*, Youssef YI1, El Zeedy SA2, Ibrahim SM2 and Al Baroudi BM1

1Department of Poultry and Rabbit Diseases, Faculty of Veterinary Medicine, Cairo University, Egypt

2 Department of Genetic Engineering, Veterinary Serum & Vaccine Research Institute (VSVRI), Agricultural Research Center (ARC), Cairo, Egypt

*Corresponding Author:
Shakal MA
Department of Poultry and Rabbit Diseases
Faculty of Veterinary Medicine
Cairo University, Egypt
Tel: 00201003939101
E-mail: [email protected]

Received Date: October 09, 2013; Accepted Date: November 19, 2013; Published Date: November 25, 2013

Citation: Shakal MA, Youssef YI, El Zeedy SA, Ibrahim SM, Al Baroudi BM (2013) Surveillance on Avian Influenza H5N1 and H9N2 Subtypes In Egypt 2012-2013. Poult Fish Wildl Sci 2:111. doi: 10.4172/2375-446X.1000111

Copyright: © 2013 Shakal MA, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.



Surveillance on Avian Influenza Virus in Egypt during 2012 - 2013 was undertaken to update the epidemiology of avian influenza virus infections among poultry flocks in Egypt. A total of 148 commercial chicken farms were tested by single-plex RT-PCR for direct detection of avian influenza viruses using type A specific nucleoprotein (NP) gene primer sets. The results revealed 5 positive chicken flocks infected with type-A AIV. These viruses were successfully isolated and propagated in SPF eggs. H5N1/HI test was positive only for three chicken flocks. Subtype specific (RTPCR) revealed 3 positive H5 chicken flocks (2%) and 2 positive H9 chicken flocks (1.35%). All examined chicken flocks were negative for H7 subtype. Multiplex RT-PCR using H5, H7 and H9 subtypes specific primers confirmed these results. This shows the continuous co-circulation of the AIV H5 and H9 in commercial chicken flocks in Egypt complicating the respiratory problems in affected flocks.


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