Survey of Leishmania Infantum Chagasi in Wild and Domestic Animals in Urban Area and Atlantic Rainforest Fragment in Northeast, Brazil
|Andréa P. da Costa1, Juliana I. G. da S. Ferreira1, Gislene F. da S. R. Fournier2, Marcos G. Lopes1, Diego Ramirez3, Igor da C. L. Acosta1,
Julia T. R. de Lima1, Marcelo B. Labruna1, Solange M. Gennari1 and Arlei Marcili1*
|1Departamento de Medicina Veterinária Preventiva e Saúde Animal, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, SP, Brasil|
|2Departamento de Parasitologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, SP, Brasil|
|3Laboratório Especial de Coleções Zoológicas, Instituto Butantan, São Paulo, SP, Brasil|
|*Corresponding Author :||Arlei Marcili
Departamento de Medicina Veterinária Preventiva e Saúde Animal
Faculdade de Medicina Veterinária e Zootecnia
Universidade de São Paulo, SP, Brasil
Tel: +55 11 30911446
Fax: +55 11 30917928
E-mail: [email protected]
|Received April 26, 2014; Accepted June 06, 2014; Published July 05, 2014|
|Citation: Costa AP, Ferreira JLGS, Fournier GFSR, Lopes MG, Ramirez D, et al. (2014) Survey of Leishmania Infantum Chagasi in Wild and Domestic Animals in Urban Area and Atlantic Rainforest Fragment in Northeast, Brazil. J Biodivers Biopros Dev 1:120. doi:10.4172/2376-0214.1000120|
|Copyright: © 2014 Costa AP, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.|
Objective: Survey of Leishmania infantum chagasi in domestic and wild mammals in urban area and a Biological Reserve in Natal, Rio Grande do Norte, Brazil.
Methods: Domestic and wild mammals were captured in Brazilian northeast Atlantic Rainforest, Rio Grande do Norte state. Serological and parasitological studies were conducted; Leishmania isolates were positioned in phylogeny based on small subunit rDNA (SSU rDNA) and glycosomal-3-phosphate dehydrogenase (gGAPDH) gene sequences.
Results: Blood samples were collected from 138 wild and domestic mammals, comprising 66 dogs, 52 cats and 20 marsupials. Antibodies were found in 27 dogs (40.91%), two cats (3.85%) and one Didelphis albiventris (5%). The cultures of popliteal lymph node aspirates were positive in nine dogs (13.64%) and from seven (10.61%) the parasite was isolated and cryopreserved. All isolates were positioned in phylogeny based on SSU rDNA and gGAPDH in the same branch with L. infantum chagasi.
Conclusions: The proximity of the forest fragment with humans and their domestic animals provide interference in the health of wild animals. Measures to control the population of feral cats and environmental conservation should be implemented due the importance of visceral leishmaniasis.