Synchrotron Imaging of Ovaries Ex Vivo
|Upekha Basnayaka1, Dean Chapman2, Gregg Adams DVM3, Tomasz Wysokinski4, George Belev4, Rani Kanthan5, Rajni Chibbar5, Robert Lewis4, Naoto Yagi6, Kentaro Uesugi6, Masato Hoshino6 and Angela Baerwald1*|
|1Department of Obstetrics, Gynecology and Reproductive Sciences, College of Medicine, University of Saskatchewan, Saskatchewan, Canada|
|2Department of Anatomy and Cell Biology, College of Medicine, University of Saskatchewan, Saskatchewan, Canada|
|3Department of Veterinary Biomedical Sciences, Western College of Veterinary Medicine, University of Saskatchewan, Saskatchewan, Canada|
|4Canadian Light Source, Saskatchewan, Canada|
|5Department of Pathology, College of Medicine, University of Saskatchewan, Saskatchewan, Canada|
|6Japan Synchrotron Radiation Research Institute, SPring-8/JASRI, 1 Hyogo Prefecture, Japan|
|Corresponding Author :||Angela Baerwald
Department of Obstetrics
Gynecology and Reproductive Sciences
Royal University Hospital, 103 Hospital Drive
Saskatoon, SK S7N 0W8, Canada
E-mail: [email protected]
|Received December 22, 2015; Accepted February 03, 2016; Published February 10, 2016|
|Citation: Basnayaka U, Chapman D, Gregg Adams DVM, Wysokinski T, Belev G, et al. (2016) Synchrotron Imaging of Ovaries Ex Vivo. J Fertil In Vitro IVF Worldw Reprod Med Genet Stem Cell Biol 4:169. doi:10.4172/2375-4508.1000169|
|Copyright: © 2016 Basnayaka U, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.|
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Background: Conventional 2-dimensional ultrasonography is limited in its ability to detect ovarian microanatomy. The objective of this study was to determine if biomedical synchrotron techniques would be effective for imaging ovarian microanatomy, including ovarian follicles, corpora lutea, and the oocyte.
Methods: A prospective, observational study was conducted at the Canadian Light Source to compare ovarian imaging ex vivo using propagation-based computed tomography (PB-CT) synchrotron imaging, ultrasonography, and histology. Bovine (n=4) and human (n=4) ovaries were imaged fresh or formalin-fixed. The effectiveness of Talbot grating interferometry computed tomography (TGI-CT) synchrotron imaging to image preserved bovine (n=1) and human (n=1) ovaries was evaluated at the SPring-8 synchrotron, Japan.
Results: All antral follicles ≥ 2 mm and corpora lutea detected with ultrasonography were identified with PBCT. Mean follicle and luteal diameters did not differ among PB-CT, ultrasonography, and histology. The smallest follicle detected was superior with PB-CT (0.9 ± 0.4 mm) than ultrasonography (2.2 ± 0.2 mm, P<0.05). PB-CT, but not ultrasonography, allowed the detection of follicle wall cell layers (P<0.05). TGI-CT provided greater contrast for evaluating follicles, corpora lutea, and vasculature than PB-CT and ultrasonography. High contrast spherical structures resembling cumulus oocyte complexes were detected with PB-CT and TGI-CT; oocytes were only detected with TGI-CT.
Conclusion: PB-CT was as effective as ultrasonography for measuring follicle and luteal diameters and superior to ultrasonography for visualizing follicles <2 mm, follicle wall cell layers, and cumulus oocyte complexes. TGI-CT appears to provide the greatest resolution for imaging ovarian anatomy compared to ultrasonography and PB-CT. Phase contrast CT Synchrotron can be used as a model for developing high resolution tools for imaging human ovaries.