alexa Synchrotron Imaging of Ovaries Ex Vivo
ISSN: 2375-4508

Journal of Fertilization: In Vitro - IVF-Worldwide, Reproductive Medicine, Genetics & Stem Cell Biology
Open Access

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article

Synchrotron Imaging of Ovaries Ex Vivo

Upekha Basnayaka1, Dean Chapman2, Gregg Adams DVM3, Tomasz Wysokinski4, George Belev4, Rani Kanthan5, Rajni Chibbar5, Robert Lewis4, Naoto Yagi6, Kentaro Uesugi6, Masato Hoshino6 and Angela Baerwald1*
1Department of Obstetrics, Gynecology and Reproductive Sciences, College of Medicine, University of Saskatchewan, Saskatchewan, Canada
2Department of Anatomy and Cell Biology, College of Medicine, University of Saskatchewan, Saskatchewan, Canada
3Department of Veterinary Biomedical Sciences, Western College of Veterinary Medicine, University of Saskatchewan, Saskatchewan, Canada
4Canadian Light Source, Saskatchewan, Canada
5Department of Pathology, College of Medicine, University of Saskatchewan, Saskatchewan, Canada
6Japan Synchrotron Radiation Research Institute, SPring-8/JASRI, 1 Hyogo Prefecture, Japan
Corresponding Author : Angela Baerwald
Department of Obstetrics
Gynecology and Reproductive Sciences
Royal University Hospital, 103 Hospital Drive
Saskatoon, SK S7N 0W8, Canada
Tel: 1-306-844-1193
Fax: 1-306-844-8040
E-mail: [email protected]
Received December 22, 2015; Accepted February 03, 2016; Published February 10, 2016
Citation: Basnayaka U, Chapman D, Gregg Adams DVM, Wysokinski T, Belev G, et al. (2016) Synchrotron Imaging of Ovaries Ex Vivo. J Fertil In Vitro IVF Worldw Reprod Med Genet Stem Cell Biol 4:169. doi:10.4172/2375-4508.1000169
Copyright: © 2016 Basnayaka U, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Related article at Pubmed, Scholar Google

 

 

Abstract

Background: Conventional 2-dimensional ultrasonography is limited in its ability to detect ovarian microanatomy. The objective of this study was to determine if biomedical synchrotron techniques would be effective for imaging ovarian microanatomy, including ovarian follicles, corpora lutea, and the oocyte.

Methods: A prospective, observational study was conducted at the Canadian Light Source to compare ovarian imaging ex vivo using propagation-based computed tomography (PB-CT) synchrotron imaging, ultrasonography, and histology. Bovine (n=4) and human (n=4) ovaries were imaged fresh or formalin-fixed. The effectiveness of Talbot grating interferometry computed tomography (TGI-CT) synchrotron imaging to image preserved bovine (n=1) and human (n=1) ovaries was evaluated at the SPring-8 synchrotron, Japan.

Results: All antral follicles ≥ 2 mm and corpora lutea detected with ultrasonography were identified with PBCT. Mean follicle and luteal diameters did not differ among PB-CT, ultrasonography, and histology. The smallest follicle detected was superior with PB-CT (0.9 ± 0.4 mm) than ultrasonography (2.2 ± 0.2 mm, P<0.05). PB-CT, but not ultrasonography, allowed the detection of follicle wall cell layers (P<0.05). TGI-CT provided greater contrast for evaluating follicles, corpora lutea, and vasculature than PB-CT and ultrasonography. High contrast spherical structures resembling cumulus oocyte complexes were detected with PB-CT and TGI-CT; oocytes were only detected with TGI-CT.

Conclusion: PB-CT was as effective as ultrasonography for measuring follicle and luteal diameters and superior to ultrasonography for visualizing follicles <2 mm, follicle wall cell layers, and cumulus oocyte complexes. TGI-CT appears to provide the greatest resolution for imaging ovarian anatomy compared to ultrasonography and PB-CT. Phase contrast CT Synchrotron can be used as a model for developing high resolution tools for imaging human ovaries.

Keywords

Share This Page

Additional Info

Loading
Loading Please wait..
 
Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri, Food, Aqua and Veterinary Science Journals

Dr. Krish

[email protected]

1-702-714-7001 Extn: 9040

Clinical and Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemical Engineering and Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001 Extn: 9040

Earth & Environmental Sciences

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

General Science and Health care Journals

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics and Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001 Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Informatics Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Material Sciences Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Mathematics and Physics Journals

Jim Willison

[email protected]

1-702-714-7001 Extn: 9042

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001 Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

John Behannon

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001 Extn: 9042

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords