Synthesis and Evaluation of Tween 85-LPEI Copolymers for Gene Transfection In vitro and In vivo
Mingxing Wang*, Bo Wu, Jay D Tucker, Peijuan Lu, Sapana N Shah, Shante Wade and Qilong Lu
Department of Neurology, McColl-Lockwood Laboratory for Muscular Dystrophy Research, Neuromuscular/ALS Center, Carolinas Medical Center, 1000 Blythe Blvd. Charlotte, NC 28231, USA
- *Corresponding Author:
- Mingxing Wang
Department of Neurology
McColl- Lockwood Laboratory for Muscular Dystrophy Research
Neuromuscular/ALS Center,Carolinas Medical Center
1000 Blythe Blvd. Charlotte, NC 28231, USA
Email: [email protected]
Received Date: August 13, 2014; Accepted Date: September 18, 2014; Published Date: September 28, 2014
Citation: Wang M, Wu B, Tucker JD, Lu P, Shah SN, et al. (2014) Synthesis and Evaluation of Tween 85-LPEI Copolymers for Gene Transfection In vitro and In vivo. J Nanomed Nanotechnol 5:228 doi:10.4172/2157-7439.1000228
Copyright: © 2014 Wang M, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
A series of cationic amphiphlic copolymers constructed from Tween 85 and low molecular weight (Mw) polyethyleneimene (LPEI) were prepared in ambient conditions and characterized. The Mws of these copolymers ranged from around 5,000 to 25,000 Da and PEI content from 8.25% to 20.91%. The new copolymers condensed DNA efficiently with particles size below 200 nm at the weight ratio 5 of polymer/pDNA, and were stable in the presence of Serum or Heparin. The introduction of Tween 85 led to a significant increase in the cellular uptake of complexes with higher transfection efficiency in CHO, C2C12, and HSkM cell lines, but without increase in toxicity compared with the parent LPEI. The best formulation for pDNA delivery produced transgene expression efficiency 5, 20-fold of PEI 25k in vitro and in mdx mice in vivo, respectively. There is no obvious muscle damage with these new copolymers at the injection sites. These results indicated that the presence of more hydrophobic groups within the new polymers is associated with higher transfection efficiency (TE), and the higher PEI content and Mw of polymers show higher TE with relatively higher toxicity. The Tween 85 modified LPEI could be a potentially safe and effective polymeric carriers for gene/drug delivery.