Journal of Analytical & Bioanalytical Techniques
Open Access

Research Article

Synthesis of Surface Molecularly Imprinting Polymers for Methylphenidate and its Application in Separating Methylphenidate

Mehdi Rajabnia Khansari^1,2*, Sara Shahreza⁴, Azam Rezvanirad¹, Amin Nikavar², Shahrzad Bikloo³ and Bahareh Sadat Yousefsani⁵

¹Faculty of Pharmacy, Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran

²School of Chemical Engineering, Research Center, Iran University of Science and Technology, Tehran, Iran

³Lorstan University of Medical Sciences, Research Center, Khoramabad, Iran

⁴Department of Nanobiotechnology, Tarbiat Modates University, Tehran, Iran

⁵Department of Pharmacodynamy and Toxicology, School of Pharmacy, Pharmaceutical Research Center, Mashhad University of Medical Sciences, Mashhad, Iran

*Corresponding Author:

Mehdi Rajabnia Khansari
Research Center, Faculty of Pharmacy
Shahid Beheshti University of Medical Sciences
Tehran, Iran
E-mail: rajabniamahdi@yahoo.com

Received date: February 11, 2017; Accepted date: February 23, 2017; Published date: February 28, 2017

Citation: Khansari MR, Shahreza S, Rezvanirad A, Nikavar A, Bikloo S, et al. (2017) Synthesis of Surface Molecularly Imprinting Polymers for Methylphenidate and its Application in Separating Methylphenidate. J Anal Bioanal Tech 8:351. doi: 10.4172/2155-9872.1000351

Copyright: © 2017 Khansari MR, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

In this study, a novel approach is proposed for determination of methylphenidate in biological fluids. In this method molecularly imprinted solid-phase extraction (MISPE), as the sample extraction technique, combined with high-performance liquid chromatography (HPLC) is used. The water-compatible molecularly imprinted polymers (MIPs) were prepared using methacrylic acid as functional monomer, ethylene glycol dimethacrylate as cross-linker, Hexane as porogen and methylphenidate as template molecule. Extraction of methylphenidate from human serum was carried out using a novel imprinted polymer as the solid-phase extraction (SPE). Various parameters affecting the extraction efficiency of the polymer were evaluated. Also, the optimal conditions for the MIP cartridges were studied. The limit of detection (LOD) and limit of quantification (LOQ) for methylphenidate in serum samples were 1.3 and 10 ng mL-1, respectively. The recoveries for serum samples were higher than 92%.

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