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ISSN: 2155-9899

Journal of Clinical & Cellular Immunology
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Review Article

Synthetic Oligonucleotide Probes for Detection of Autoimmune Antibodies

Kira Astakhova*
Nucleic Acid Center, Department of Physics, Chemistry and Pharmacy, University of Southern Denmark, Campusvej 55, DK-5230 Odense M, Denmark
Corresponding Author : Kira Astakhova
Nucleic Acid Center, Department of Physics
Chemistry and Pharmacy, University of Southern Denmark
Campusvej 55, DK-5230 Odense M, Denmark
Tel: (+45) 6550 2523
Fax: (+45) 6615 8780
E-mail: [email protected]
Received January 20, 2014; Accepted March 17, 2014; Published March 24, 2014
Citation: Astakhova K (2014) Synthetic Oligonucleotide Probes for Detection of Autoimmune Antibodies. J Clin Cell Immunol 5:199. doi: 10.4172/2155-9899.1000199
Copyright: © 2014 AstakhovaK, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use,distribution, and reproduction in any medium, provided the original author and source are credited.
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Abstract

In this review, applications of synthetic oligonucleotide probes in diagnostics and studies of autoimmune antibodies against double-stranded DNA (anti-dsDNA) are described. As outlined herein, synthetic oligonucleotides and monoclonal antibodies provide appealing opportunities to develop standard simple assays for detection of antidsDNAs. Recent examples of anti-dsDNA detection using synthetic nucleic acid antigens include those applying surface plasmon resonance (SPR), antibody microarrays and homogeneous, or all-in-solution, detection with fluorescently-labelled probes. Since sequences of the applied nucleic acid antigens and monoclonal antibodies are known, the major benefit of these assays is the ability for the first time to clearly define the structural factors that govern the formation and stability of anti-dsDNA complexes. This is an essential first step toward understanding immune-complex mediated tissue injury in autoimmune conditions such as systemic lupus erythematosus (SLE) and arthritis, which involve production of pathogenic anti-dsDNAs. Moreover, synthetic nucleic acids and monoclonal antibodies have been demonstrated as key tools for the development of novel nucleic acid sensors for serotyping, along with studies of specificity and avidity of the antibody-dsDNA binding process. Among other antigens, fluorescent oligonucleotides prepared by click chemistry between novel alkyne-modified locked nucleic acid (LNA) strands and a series of fluorescent azides are proved to be very promising tools for efficient homogeneous detection of anti-dsDNAs.

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