Technical Evaluation of Antioxidant ActivityAline Augusti Boligon1*, Michel Mansur Machado2 and Margareth Linde Athayde1
- *Corresponding Author:
- Aline Augusti Boligon
Phytochemical Research Laboratory
Department of Industrial Pharmacy
Federal University of Santa Maria, Build 26
Room 1115, Santa Maria, RS 97105-900, Brazil
Tel: +55 55 32209618
Fax: +55 55 32208248
E-mail: [email protected]
Received date: May 24, 2014; Accepted date: June 30, 2014; Published date: July 02, 2014
Citation: Boligon AA, Machado MM, Athayde ML (2014) Technical Evaluation of Antioxidant Activity. Med chem 4:517-522. doi: 10.4172/2161-0444.1000188
Copyright: © 2014 Boligon AA, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
There are several in vitro methods for evaluating antioxidant activity. This review article gives information regarding different methods that are used to measure the antioxidant defense system In addition; the advantages and shortcomings, as well as the specific cases of their application also are demonstrated. The chemical principles of methods based on biological oxidants comprise superoxide radicals scavenging (O2 ·-); hydroxyl radical scavenging (HO.); hydrogen peroxide scavenging (H2O2); peroxyl radical scavenging (ROO.) and nitric oxide scavenging (NO.). Among the non-biological testing can highlight scavenging of 2,2-diphenyl-1-picrylhydrazyl radical (DPPH• assay) and scavenging of 2,2-azinobis-(3-ethylbenzothiazoline-6-sulphonate) radical cation (ABTS assay). Furthermore, thiobarbituric acid reactive substances (TBARS) and protein carbonyl assays also have been described. This article will be a comprehensive ready reference for those who are interested on antioxidant study.