Test Characteristics of the AnigenÃÂ® Rapid AIV Ag Test (Avian Influenza Type A Rapid Antigen Test) in Ducks in VietnamLeo Loth1, Pham Thanh Long2, Nguyen Tung3, Nguyen Hoang Dang3 and Scott Newman1
- *Corresponding Author:
- Leo loth
Food and Agriculture Organization of the United Nations, Hanoi, Vietnam
E-mail: [email protected]
Received date: September 23, 2014; Accepted date: February 23, 2015; Published date: February 28, 2015
Citation: Loth L, Long PT, Tung N, Dang NH, Newman S (2015) Test Characteristics of the Anigen® Rapid AIV Ag Test (Avian Influenza Type A Rapid Antigen Test) in Ducks in Vietnam. Virol-mycol 4:140. doi:10.4172/2161-0517.1000140
Copyright: © 2015 Loth et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
The current study evaluated the test characteristics of a commercially available rapid antigen test (The Anigen® Rapid AIV Ag test) for Highly Pathogenic Avian Influenza (HPAI). Diagnostic specimens were collected from 175 ducks at markets and under controlled laboratory conditions in Vietnam. The relative diagnostic test sensitivity and the relative diagnostic test specificity were calculated by using real-time Reverse Transcription Polymerase Chain Reaction tests as the confirmatory diagnosis. The Anigen® Rapid AIV Ag test had a relative diagnostic sensitivity of 0.61 (95% confidence interval [CI] 0.45-0.75) and a relative diagnostic specificity of 0.98 (95% CI: 0.94–0.99). The result show that the Anigen® test can detect, with limited sensitivity, HPAI H5N1 clades circulating in Vietnam. The test can aid by making a differential diagnosis in sick or dying chickens, ducklings and adult ducks. Due to the limited sensitivity more birds may need to be tested to increase flock sensitivity. As flock prevalence of virus shedding ducks in Vietnam is low, this test is not suitable to reliably and quickly detecting Influenza A virus from apparently healthy ducks.