Th1, Th2 Serum Cytokines and Spleen White Pulp Changes Against Preliminary L. Major Vaccine Injection and Challenge With Live L. Major Promastigotes in Balb/C Mice
|Latifynia A1,2*, Gharagozlou MJ3, Mohebali M4, Hajjaran H4 and Khansari N1|
|1Department of Immunology, Faculty of medicine, Tehran University of Medical Sciences, Tehran, Islamic Republic of Iran|
|2Research Center for Immunodeficiencies, Children’s Medical Center Hospital, Tehran University of Medical Sciences, Tehran, Islamic Republic of Iran|
|3Department of Pathobiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Islamic Republic of Iran|
|4Department of Parasitology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran|
|Corresponding Author :||Latifynia A
Department of Immunology, Faculty of medicine
Tehran University of Medical Sciences
Tehran, Islamic Republic of Iran
E-mail: [email protected]
|Received November 16, 2014; Accepted January 04, 2015; Published January 12, 2015|
|Citation: Latifynia A, Gharagozlou MJ, Mohebali M, Hajjaran H, Khansari N (2015) Th1, Th2 Serum Cytokines and Spleen White Pulp Changes Against Preliminary L. Major Vaccine Injection and Challenge With Live L. Major Promastigotes in Balb/C Mice. J Clin Cell Immunol 5:281. doi:10.4172/2155-9899.1000281|
|Copyright: © 2015 Latifynia A, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.|
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Introduction: Human Leishmaniasis is distributed worldwide and is mainly in the tropics and subtropics, with a prevalence of 12 million cases and an approximately incidence of 0.5 million cases of visceral Leishmaniasis(VL) and 1.5 million cases of cutaneous Leishmaniasis (CL). Leishmania parasites are vector-born protozoan pathogens found in tropical and subtropical regions of both the old and new world. The disease in human can be divided into cutaneous, visceral, and mucosal syndromes. The aim of this study was to conduct further studies over our new formulation of Leishmania major vaccine which experimentally had satisfactory results.
Method: For the detail of the procedure it is referred to the author’s previous publications. Briefly one hundred and twenty Balb/c mice were randomly divided into four groups as LT, LB, LBT and control groups. Groups LT, LB and LBT injected subcutaneously with the antigen and the same booster doses with a week interval. The expansion rates of the spleen white pulp size was evaluated and the levels of the serum TH1 (IFN-γ, IL-12) and TH2 (IL-4, IL-10) cytokines measured with the ELISA method.
Results: Comparing to the LT and LB groups, the LBT group had highest levels of serum IL-12, lowest levels of IL-10 and highest increase in the spleen white pulp size. Significant negative correlation was observed between IL-12 and IL-10 but not IFN-γ or IL-4.
Conclusion: The present study indicated that the LBT group which received crude cocktail Leishmania antigen plus alcoholic extract of Teucrium polium and BCG as adjuvants showed satisfactory cytokines profile comparing to groups LT and LB, since highest levels of IL-12 and lower levels of IL-10 could help the infected subjects to inhibit or eradicate the intracellular Leishmania amastigotes, and also highest increase in the spleen white pulp size which pointed to the synergistic effects of BCG and alcoholic extract of T. polium.