alexa The Chicken and Egg System for the Development of Anti-Idiotypic Vaccines | OMICS International
ISSN: 2157-7560

Journal of Vaccines & Vaccination
Open Access

Like us on:
OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article

The Chicken and Egg System for the Development of Anti-Idiotypic Vaccines

Angel Alberto Justiz Vaillant1, Patrick Eberechi Akpaka1*, Norma McFarlane-Anderson2, Monica P. Smikle3 and Wisdom Brian4

1Department of Para-Clinical Sciences, The University of the West Indies, St. Augustine, Trinidad & Tobago

2Department of Basic Medical Sciences, Faculty of Medical Sciences, University of the West Indies, Mona, Kingston, Jamaica

3Department of Microbiology, Faculty of Medical Sciences, University of the West Indies, Mona, Kingston, Jamaica

4School of Biology and BioChemistry, Medical Biology Centre, The Queen’s University of Belfast, Belfast, UK

*Corresponding Author:
Dr. Patrick Eberechi Akpaka
Department of Para-Clinical Sciences
The University of the West Indies
St. Augustine, Trinidad & Tobago
Tel: +868-736-0440
Fax: +868-663-3797
Email: [email protected], [email protected]

Received Date: January 20, 2012; Accepted Date: April 24, 2012; Published Date: April 26, 2012

Citation:Vaillant AAJ, Akpaka PE, McFarlane-Anderson N, Smikle MP, Brian W (2012) The Chicken and Egg System for the Development of Anti-Idiotypic Vaccines. J Vaccines Vaccin 3:137 doi: 10.4172/2157-7560.1000137

Copyright: © 2012 Vaillant AAJ, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

This study investigates the use of the chicken and egg system for the development of an oral HIV vaccine. Brown leghorn chickens were immunized with keyhole limpet hemocynin conjugated with a HIV-gp120 peptide (fragment 254-274). An indirect ELISA for antibodies to HIV-gp120 was used to measure anti-HIV antibody titres in the watery soluble fraction of eggs up to 14 weeks after the second week post-immunization. Over a period of 10 weeks, 3 cats were fed with the eggs from the immunized chickens and 2 cats with eggs from non-immunized chickens. An indirect enzyme linked-immunosorbent assay (ELISA) and a binding inhibition assay were used to assess the antibody response to HIV-gp120 peptide in the cat serum. The most important finding was the development of serum anti- HIV antibodies in cats fed with eggs from chickens that were positive for anti-HIV antibodies. These feline anti-HIV antibodies bound to the original HIV-gp120 peptide and also inhibited the binding of egg yolk anti-HIV antibodies to the HIV gp120 peptide, showing that the anti-HIV antibody raised in cats after feeding, was an anti-anti-idiotypic antibody. The results of this study suggest that eggs from immunized hens could be considered in the management of HIV infections.

Keywords

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri & Aquaculture Journals

Dr. Krish

[email protected]

1-702-714-7001Extn: 9040

Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001Extn: 9042

 
© 2008- 2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version