The Chicken and Egg System for the Development of Anti-Idiotypic Vaccines
- *Corresponding Author:
- Dr. Patrick Eberechi Akpaka
Department of Para-Clinical Sciences
The University of the West Indies
St. Augustine, Trinidad & Tobago
Email: [email protected], [email protected]
Received Date: January 20, 2012; Accepted Date: April 24, 2012; Published Date: April 26, 2012
Citation:Vaillant AAJ, Akpaka PE, McFarlane-Anderson N, Smikle MP, Brian W (2012) The Chicken and Egg System for the Development of Anti-Idiotypic Vaccines. J Vaccines Vaccin 3:137 doi: 10.4172/2157-7560.1000137
Copyright: © 2012 Vaillant AAJ, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
This study investigates the use of the chicken and egg system for the development of an oral HIV vaccine. Brown leghorn chickens were immunized with keyhole limpet hemocynin conjugated with a HIV-gp120 peptide (fragment 254-274). An indirect ELISA for antibodies to HIV-gp120 was used to measure anti-HIV antibody titres in the watery soluble fraction of eggs up to 14 weeks after the second week post-immunization. Over a period of 10 weeks, 3 cats were fed with the eggs from the immunized chickens and 2 cats with eggs from non-immunized chickens. An indirect enzyme linked-immunosorbent assay (ELISA) and a binding inhibition assay were used to assess the antibody response to HIV-gp120 peptide in the cat serum. The most important finding was the development of serum anti- HIV antibodies in cats fed with eggs from chickens that were positive for anti-HIV antibodies. These feline anti-HIV antibodies bound to the original HIV-gp120 peptide and also inhibited the binding of egg yolk anti-HIV antibodies to the HIV gp120 peptide, showing that the anti-HIV antibody raised in cats after feeding, was an anti-anti-idiotypic antibody. The results of this study suggest that eggs from immunized hens could be considered in the management of HIV infections.