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The Creation of Electrospun Nanofibers from Platelet Rich Plasma | OMICS International | Abstract
ISSN: 2157-7552

Journal of Tissue Science & Engineering
Open Access

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Research Article

The Creation of Electrospun Nanofibers from Platelet Rich Plasma

Patricia S Wolfe1#, Scott A Sell1,2#, Jeffery J Ericksen2,3, David G Simpson4 and Gary L Bowlin1*

1Department of Biomedical Engineering, Virginia Commonwealth University, 401 W. Main St., Richmond, VA 23284, USA

2Physical Medicine and Rehabilitation Service, Hunter Holmes McGuire VA Medical Center, 1201 Broad Rock Blvd., Richmond, VA 23249, USA

3Department of Physical Medicine and Rehabilitation, Virginia Commonwealth University, Richmond, VA 23298, USA

4Department of Anatomy and Neurobiology, Virginia Commonwealth University, Richmond, VA 23298, USA

#Authors contributed equally

Corresponding Author:
Dr. Gary L. Bowlin
Department of Biomedical Engineering
Virginia Commonwealth University, P.O. Box 843067
Richmond, VA 23284
Email: [email protected]

Received date: March 08, 2011; Accepted date: May 18, 2011; Published date: May 20, 2011

Citation: Wolfe PS, Sell SA, Ericksen JJ, Simpson DG, Bowlin GL (2011) The Creation of Electrospun Nanofibers from Platelet Rich Plasma. J Tissue Sci Eng 2:107. doi:10.4172/2157-7552.1000107

Copyright: © 2011 Wolfe PS, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

Activated platelet rich plasma (a PRP) contains supra physiologic amounts of autologous growth factors and cytokines known to enhance wound healing and tissue regeneration. Here we report the first results of electro spinning nanofibers from a PRP to create fibrous scaffolds that could be used for various tissue engineering applications. Human platelet rich plasma (PRP) was created, activated by a freeze-thaw-freeze process, and lyophilized to form a powdered preparation rich in growth factors (PRGF). It was dissolved in 1,1,1,3,3,3-hexafluoro-2-propanol (HFP) at different concentrations to form fibers with average diameters of 0.3 ? 3.6 ?m. A sustained release of protein from the PRGF scaffolds was demonstrated up to 35 days, and cell interactions with the PRGF scaffolds confirmed cell infiltration after just 3 days. As electro spinning is a simple process, and PRGF contains naturally occurring growth factors in physiologic ratios, creating nanofibrous structures from PRGF has the potential to be beneficial for a variety of tissue engineering applications.

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