The Creation of Electrospun Nanofibers from Platelet Rich Plasma
- Corresponding Author:
- Dr. Gary L. Bowlin
Department of Biomedical Engineering
Virginia Commonwealth University, P.O. Box 843067
Richmond, VA 23284
Email: [email protected]
Received date: March 08, 2011; Accepted date: May 18, 2011; Published date: May 20, 2011
Citation: Wolfe PS, Sell SA, Ericksen JJ, Simpson DG, Bowlin GL (2011) The Creation of Electrospun Nanofibers from Platelet Rich Plasma. J Tissue Sci Eng 2:107. doi:10.4172/2157-7552.1000107
Copyright: © 2011 Wolfe PS, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Activated platelet rich plasma (a PRP) contains supra physiologic amounts of autologous growth factors and cytokines known to enhance wound healing and tissue regeneration. Here we report the first results of electro spinning nanofibers from a PRP to create fibrous scaffolds that could be used for various tissue engineering applications. Human platelet rich plasma (PRP) was created, activated by a freeze-thaw-freeze process, and lyophilized to form a powdered preparation rich in growth factors (PRGF). It was dissolved in 1,1,1,3,3,3-hexafluoro-2-propanol (HFP) at different concentrations to form fibers with average diameters of 0.3 ? 3.6 ?m. A sustained release of protein from the PRGF scaffolds was demonstrated up to 35 days, and cell interactions with the PRGF scaffolds confirmed cell infiltration after just 3 days. As electro spinning is a simple process, and PRGF contains naturally occurring growth factors in physiologic ratios, creating nanofibrous structures from PRGF has the potential to be beneficial for a variety of tissue engineering applications.