alexa The Discrepancy of the Minimum Inhibitory Concentration Results for Methicillin-Resistant Staphylococcus aureus by Various Measurement Methods | OMICS International | Abstract
ISSN: 2165-7548

Emergency Medicine: Open Access
Open Access

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article

The Discrepancy of the Minimum Inhibitory Concentration Results for Methicillin-Resistant Staphylococcus aureus by Various Measurement Methods

Yuka Kitano1, Shigeki Fujitani1, Haruaki Wakatake1, Machi Yanai1, Sari Umekawa1, Yosuke Homma2, Yasuhiko Taira1*

1Department of Emergency and Critical Care Medicine, St. Marianna University Hospital, Kawasaki City, Japan

2Department of Emergency Medicine, Tokyo Bay Urayasu Ichikawa Medical Center, Japan

*Corresponding Author:
Yasuhiko Taira, MD
2-16-1 Sugao Miyamae-ku
Kawasaki City
Kanagawa Prefecture, Japan-216-8511
Tel: +81-44-977-8111 ext 3931
Fax: +81-44-979-1522
E-mail: [email protected]

Received Date: September 21, 2015; Accepted Date: November 12, 2015; Published Date: November 19, 2015

Citation: Kitano Y, Fujitani S, Wakatake H, Yanai M, Umekawa S, et al. (2015) The Discrepancy of the Minimum Inhibitory Concentration Results for Methicillin-Resistant Staphylococcus aureus by Various Measurement Methods: A Comparison of Etest® and Microdilution Methods for Vancomycin, Teicoplanin, Linezolid, Daptomycin and Quinupristin-Dalfopristin. Emergency Med 5:286. doi:10.4172/2165-7548.1000286

Copyright: © 2014 Kitano Y, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

Background: It has been postulated that there exists a discrepancy of methicillin-resistant Staphylococcus aureus (MRSA) minimum inhibitory concentration (MIC) results from various measurement methods. The association between higher MRSA MIC (MIC ≥ 2 μg/ml) and worse clinical outcome has been previously reported. Therefore, it is clinically essential to investigate whether such a discrepancy exists between the different MIC measurement methods.
Methods: From November 2009 to March 2011, 55 MRSA isolates were prospectively obtained at two emergency departments in Japan. The MIC of the isolates were measured by Etest® and five broth microdilution (BMD) methods, namely Eiken®, MicroScan® prompt method, MicroScan® turbidity method, Phoenix® and Vitek2® system, respectively. The MIC results of vancomycin, teicoplanin, linezolid, daptomycin and quinupristin-dalfopristin (Q-D) were evaluated. Statistical analysis was performed using the Wilcoxon signed rank test and Bland-Altman’s analysis.
Results: There was a tendency of constant and significant discrepancy of MIC results of anti-MRSA antibiotics between Etest® and the BMD methods for MRSA isolates. The averages of the vancomycin MIC were 1.86 μg/ ml in Etest® and 0.74 μg/ml in the Phoenix® method (p<0.01), respectively. For teicoplanin, they were 1.86 μg/ml and 0.60 μg/ml (p<0.01), and for linezolid they were 2.55 μg/ml and 1.18 μg/ml (p<0.01) with respect to Etest® and the Phoenix® method, respectively. Among the BMD methods, however, the MicroScan® prompt method and the MicroScan® turbidity method had less discrepancy from Etest® for vancomycin MIC measurement.
Conclusion: The MIC measured by various BMD methods tended to show consistently lower results compared to those measured by Etest®. Among the BMD methods, however, the MicroScan® prompt method and the MicroScan® turbidity method had less discrepancy from Etest® for vancomycin MIC measurement.

Keywords

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2018-19
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri & Aquaculture Journals

Dr. Krish

[email protected]

1-702-714-7001Extn: 9040

Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001Extn: 9042

 
© 2008- 2018 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version