The Effect of Aluminium on the Morphologic Appearance, Viability and Phagocytic Activity of ARPE-19 Cells
|Gertrud Haas, Claus Zehetner, Stefan Huber, Yvonne Nowosielski, Nikolaos Bechrakis and Josef Troger*|
|Department of Ophthalmology and Optometry, University Clinics, Medical University of Innsbruck, Anichstraße 35, 6020 Innsbruck, Austria|
|Corresponding Author :||Josef Troger
Department of Ophthalmology and Optometry
Medical University of Innsbruck, Anichstraße 35
6020 Innsbruck, Austria
E-mail: [email protected]
|Received November 24, 2014; Accepted December 22, 2014; Published December 26, 2014|
|Citation: Haas G, Zehetner C, Huber S, Nowosielski Y, Bechrakis N, et al. (2014) The Effect of Aluminium on the Morphologic Appearance, Viability and Phagocytic Activity of ARPE-19 Cells. J Clin Exp Ophthalmol 5:383. doi: 10.4172/2155-9570.1000383|
|Copyright: © 2014 Troger J, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.|
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Purpose: Aluminium is known to have toxic effects on the central nervous system. We wanted to explore the effects of aluminium on cultured ARPE-19 cells, in particular, changes in the morphologic appearance, viability and in the phagocytic activity of these cells.
Methods: After addition of different concentrations of aluminium to the cell cultures, cellular morphology was evaluated by photomicrographs; viability was determined by mitochondrial activity measurement and phagocytosis by uptake of europium-labeled FluoSpheres.
Results: Pretreatment of the cells with aluminium led to the formation of clots in the cell culture and there was a relative weak dose-dependent decrease in viability. However, phagocytic activity was severely impaired at each concentration with a peak decrease of 92.45% (± 8.21) at 1000 μmol.
Conclusions: Exposure to aluminium occurs mainly through contaminated food and beverages. Given that sufficient concentrations accumulate in the RPE, inhibition of the phagocytic activity of RPE cells might represent a novel important side effect of this metal. Although no conclusions can be drawn from in vitro results on the effect in vivo, it seems that caution is recommended with consumption of food with high concentrations of aluminium. The reduced viability of RPE cells, however, is clinically less relevant since the effect was relative weak in vitro.