alexa The Effect of Arvanil on Prostate Cancer Cells Studied by Whole Cell High Resolution Magic Angle Spinning NMR
ISSN: 2329-6798

Modern Chemistry & Applications
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Research Article

The Effect of Arvanil on Prostate Cancer Cells Studied by Whole Cell High Resolution Magic Angle Spinning NMR

Wei Li* and Bob M Moore II*
Department of Pharmaceutical Sciences, College of Pharmacy, The University of Tennessee Health Science Center, Memphis, TN 38163, USA
Corresponding Authors : Wei Li
Department of Pharmaceutical Sciences, College of Pharmacy
The University of Tennessee Health Science Center, Memphis
TN 38163, 847 Monroe Avenue, Room 327B, Memphis, TN 38163, USA
Tel: +1-901-448-7532
Fax: +1-901-448-6828
E-mail: [email protected]
  Bob M Moore II
847 Monroe Avenue, Room 327D, Memphis, 38163, USA
Tel: +1-901-448-6085
Fax: +1-901-448-6828
E-mail: [email protected]
Received January 29, 2014; Accepted February 21, 2014; Published February 24, 2014
Citation: Li W, Moore II BM (2014) The Effect of Arvanil on Prostate Cancer Cells Studied by Whole Cell High Resolution Magic Angle Spinning NMR. Mod Chem appl 2:119. doi: 10.4172/2329-6798.1000119
Copyright: © 2014 Li W, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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Abstract

The transient receptor potential cation channel subfamily V member 1 (TRPV1) has recently gained attention as a potential target for the development of novel antineoplastic agents. It has been reported that the TRPV1 agonist arvanil has effective antiproliferative effects in studies using human breast cancer cells lines. In an extension of this research we have evaluated the IC50 values of arvanil in the prostate cancer cell lines PPC-1 (primary) and TSU (metastatic). Both TSU and PPC-1 cell lines are sensitive to treatment with arvanil. This result prompted our investigations into the changes in “cell metabolism” associated with prostate cancer progression and the effect of arvanil treatment. To this end, we have employed High Resolution Magic-Angle Spinning (HR-MAS) NMR spectroscopy on whole cells to determine the differences in the relative amount of cell metabolites and changes in small molecule metabolism following treatment of the TSU and PPC-1 cells with arvanil. We evaluated and confirmed that the existing “biomarkers” such as elevated tCho and decreased citrate in prostate cancer are well correlated with prostate cancer progression. In addition, metastatic TSU cells also contain elevated level of lactate and glutamine, and contain much less creatine. Upon treatment with arvanil, a number of biomolecules were found to undergo changes in intracellular levels during apoptosis. These data will potentially permit the further characterization of signaling pathways associated with TRPV1 activation as well as identifying new targets for the development of novel antineoplastic agents.

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