The Effect of Biodegradable Scaffolds for Osteochondral Repair on the Immobilized Rabbit Knee
- *Corresponding Author:
- Yung-Chang Lin
Department of Veterinary Medicine
National Chung Hsing University, No.250
KuoKuang Rd., South Dist
Taichung City 402, Taiwan
E-mail: [email protected], [email protected]
Received date: December 07, 2013; Accepted date: December 30, 2013; Published date: January 07, 2014
Citation: Lin CC, Chang NJ, Yeh ML, Li CF, Lin YC (2014) The Effect of Biodegradable Scaffolds for Osteochondral Repair on the Immobilized Rabbit Knee. Surgery Curr Res 4:158. doi:10.4172/2161-1076.1000158
Copyright: © 2014 Lin CC, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Background: An initial constrained weight-bearing period achieved via immobilization (Imm) is recommended during osteochondral regeneration to avoid tissue re-injury. However, remedying osteochondral defects with a scaffoldbased treatment and Imm intervention has yet to be studied in osteochondral defect models. Therefore, we investigated the combined outcome of short-term Imm on osteochondral defects with and without porous poly (lactic-co-glycolic acid) (PLGA) scaffold implants.
Methods: Twenty-four rabbits were divided into empty defect (ED) and PLGA-implanted (PI) groups. Each rabbit’s unilateral knee, which contained an osteochondral defect, was immobilized at full extension with a splint for 2 weeks after surgery and subsequently remobilized until sacrifice at 4 weeks or 12 weeks post surgery. The health status; gross appearance; histological analysis detailing collagen, glycosaminoglycan, and matrix metalloproteinase (MMP)- 13 expression levels; and micro-CT images of the rabbits were evaluated.
Results: At 4 weeks, the ED and PI groups both exhibited degenerative cartilage features, including an irregular joint surface, fibrous or fibrocartilage formation, decreased glycosaminoglycan (GAG) expression, and increased MMP-13 expression. However, at 12 weeks, the PI group exhibited significantly higher GAG expression, lower MMP- 13 expression, and more bony deposition matrix and trabecular bone than the ED group.
Conclusion: Our findings indicate that the short-term Imm is harmful to articular cartilage; however, PLGA grafts could provide a matrix for tissue regeneration