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The Effect of Chemokine Receptor Antagonists on the Migration of Tumour Associated Macrophages | OMICS International | Abstract

Journal of Molecular Biomarkers & Diagnosis
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Research Article

The Effect of Chemokine Receptor Antagonists on the Migration of Tumour Associated Macrophages

Emanual Michael Patelia1* and Dannamaria Gal2
1Department of Pharmacology, University of Bedfordshire, UK
2Gray Institute for Radiation Oncology and Biology, University of Oxford, UK
*Corresponding Author : Emanual Michael Patelia
Master of Science
Department of Pharmacology
University of Bedfordshire, UK
Tel: +44 1234 400400
E-mail: [email protected]
Received: November 12, 2015; Accepted: February 15, 2016; Published: February 17, 2016
Citation: Patelia EM, Gal D (2016) The Effect of Chemokine Receptor Antagonists on the Migration of Tumour Associated Macrophages. J Mol Biomark Diagn 7:273. doi:10.4172/2155-9929.1000273
Copyright: © 2016 Patelia EM, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


The macrophages especially the macrophages associated with the tumour development are the main focus for the experimental purpose. During the experiment, the RAW 264.7 mouse macrophage cell linewascultured in aseptic conditions and then polarized into the M1 and M2 phenotypes. The cytokines such as IL-4, IFN-γ, and IL-4 were used for the induction of polarization. The expected cellular markers of M2 such as CD204 and CD206 has been shown by Western blot analysis. The M1 and M2 phenotypes were evaluated for phagocyticactivity, M2 phenotype being recorded with highest relative phagocytic activity. The chemo tactic movement of RAW264.7 macrophages kept in co-cultre with B16F10 mouse melanoma cells was similar to the transmigration obtained by RANTES (CCL5) alone. The α-RANTES antibody and the CCR2 antagonist significantly reduced transmigration of macrophages in co-culture with B16F10 cells.