alexa The Effect of Green Tea Extract on Submandibular Salivary Gland of Methotrexate Treated Albino Rats: Immunohistochemical Study | OMICS International | Abstract
ISSN: 2157-7099

Journal of Cytology & Histology
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Research Article

The Effect of Green Tea Extract on Submandibular Salivary Gland of Methotrexate Treated Albino Rats: Immunohistochemical Study

Ali Sultan Al-Refai1, Ameera Kamal Khaleel2* and Shaheen Ali2*

1 Anatomy Branch - Department of Oral & Maxillofacial Surgery, College of Dentistry, Hawler Medical University, Erbil, Kurdistan Region of Iraq

2 Department of Oral Diagnosis, College of Dentistry, Hawler Medical University, Erbil, Kurdistan Region of Iraq

*Corresponding Author:
Ameera Kamal
Department of Oral Diagnosis
College of Dentistry
Hawler Medical University, Erbil
Kurdistan Region of Iraq
Tel: 009647504882164
E-mail: [email protected]
Shaheen Ali
Department of Oral Diagnosis
College of Dentistry
Hawler Medical University
Erbil, Kurdistan Region of Iraq
Tel: 009647504483301
E-mail: [email protected]

Received Date: October 29, 2013; Accepted Date: December 30, 2013; Published Date: January 01, 2014

Citation: Al-Refai AS, Khaleel AK, Ali S (2014) The Effect of Green Tea Extract on Submandibular Salivary Gland of Methotrexate Treated Albino Rats: Immunohistochemical Study. J Cytol Histol 5:212. doi:10.4172/2157-7099.1000212

Copyright: © 2014 Al-Refai AS, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

Background and objectives: Methotrexate (MTX) had been used for many years and complications usually encountered during treatment especially in cancer patients. The aim of the present study was to determine the preventive and early-stage anti cytotoxic effects of green tea on the histology of the submandibular gland of rats treated by high single dose of methotrexate.
Materials and methods: The study included 36 Albino rats. Twelve animals were used in the pilot study to find the maximum toxic dose, and the other twenty four were divided into four groups, vehicle treated control group, green tea extract treated control group (40 mg/kg/day), methotrexate treated group (80 mg/kg), and methotrexate and green tea extract treated group. Submandibular glands excisions were then performed. Histopathological examination was performed with hematoxylin-eosin, Masson’s trichrome, and PAS stains. Cell proliferation was examined using the Ki-67 antibody and anti apoptotic effect was determined based on Bcl-2 staining.
Results: In the methotrexate and green tea extract treated group a non significant difference in the Ki-67 expression (p>0.01) and a significant increase in Bcl-2 expression (p<0.01) were seen in comparison with the methotrexate treated group.
Conclusion: Green tea aqueous extract at a concentration of 40 mg/kg/day produced a protection against methotrexate induced cytotoxicity in rat submandibular gland by increasing the expression of Bcl-2.

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