alexa The Expression of Procalcitonin in the Central Nervous
ISSN: 2168-9296

Cell & Developmental Biology
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Research Article

The Expression of Procalcitonin in the Central Nervous System of the Rat Embryo

Jesus Canudas1,2*, Victor Sorribas3, Ana M Lacosta1,2 and Manuel Sarasa1,2*
1Araclon Biotech Ltd, Via Hispanidad 21–50009-Zaragoza, Spain
2Department of Anatomy, Embryology and Animal Genetics, Faculty of Veterinary, Spain
3Laboratory of Molecular Toxicology, Faculty of Veterinary, Miguel Servet 177-50013-Zaragoza, Spain
Corresponding Authors : Jesus Canudas
Araclon Biotech Ltd
Via Hispanidad 21–50009-Zaragoza, Spain
Tel: +34-876 241 666
Fax: +34-976 217 802
E-mail: [email protected]
  Manuel Sarasa
Araclon Biotech Ltd
Via Hispanidad 21–50009-Zaragoza, Spain
Tel: +34-876 241 666
Fax: +34-976 217 802
E-mail: [email protected]
Received September 12, 2013; Accepted October 17, 2013; Published October 21, 2013
Citation: Canudas J, Sorribas V, Lacosta AM, Sarasa M (2013) The Expression of Procalcitonin in the Central Nervous System of the Rat Embryo. Cell Dev Biol 2:126. doi:10.4172/2168-9296.1000126
Copyright: © 2013 Canudas J, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


Procalcitonin mRNA is abundantly synthesized in the thyroid gland in mammals. In recent years, procalcitonin or its posttranslational product calcitonin, has been identified in numerous non-thyroidal adult tissues. Nevertheless, little is known about the implications of procalcitonin in development. We have previously discovered that chick embryo expresses procalcitonin in the developing floor plate and the dorsal rhombencephalon from the early stages of embryogenesis. This finding focused our attention on investigating whether procalcitonin is present in the nervous system of mammalian embryos. In a previous study by our laboratory, the central expression of calcitonin was not found in rat embryos when isotopic deoxyoligonucleotides were used as probes for in situ hybridization analyses. In this study, the expression of procalcitonin mRNA was detected in rat embryos from 11 to 18 days post-coitum when analyzed using techniques such as RT-PCR. In situ hybridization histochemistry with riboprobes revealed the expression of procalcitonin mRNA in the roof of the diencephalon from 12 to 16 days post-coitum and this expression was quantified by Ribonuclease Protection Assay technique. The immunohistochemical analysis confirmed the presence of the protein in this region and in the roof (velum medullare) of the rhombencephalon. Notably, numerous microglial cells were present in the developing diencephalon only during the period during which the expression of procalcitonin was detected. These findings suggest roles for procalcitonin not only in diencephalon development but also in the process of microglial colonization of the central nervous system.

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