The Hypoglycaemic Effect of the Components from Pleurotus eryngii was evaluated on model In vitro and In vivoJinmei Lin1,2, Jingna Liu1, Jiaofen Lin1, Jiafu Huang1, Zhichao Lin1, Bo Leng1, Yu Xue1, Yi Su1, Liyun Yao1, Xiumin Li1, Yuanhong Zhuang1 and Yutian Pan1*
- *Corresponding Author:
- Yutian Pan
Engineering Technological Center of Mushroom Industry
Minnan Normal University, Zhangzhou, 363000, China
E-mail: [email protected]
Received Date: October 17, 2016; Accepted Date: November 15, 2016; Published Date: December 1, 2016
Citation: Lin J, Liu J, Lin J, Huang J, Lin Z, et al. (2016) The Hypoglycaemic Effect of the Components from Pleurotus eryngii was evaluated on model In vitro and In vivo. Appli Micro Open Access 2:125. doi: 10.4172/2471-9315.1000125
Copyright: © 2016 Lin J. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Four structural substances were extracted from P. eryngii, including PEE-1, PEE-2, PEE-3 and PEE-4. The chemical analysis was conducted to measure the contents of polysaccharide and protein in PEE-1, results found that they were 10.78% and 26.66% respectively. PEE-2, PEE-3 and PEE-4 were respectively identified as β-glucan, chitosan and D-glucosamine hydrochloride by FT-IR, GC, HPLC and HPLC-MS. The results showed that PEE-1, PEE-2 and PEE-3 could inhibit the activity of α-glycosidase from the rabbit isolated small intestine, while the PEE-4 had no inhibitory effect. At the same concentration, the PEE-1 showed more significantly inhibitory effects on α- glycosidase activity than Glucobay (P<0.05). The hypoglycemic effect of PEE-1 had significant differences in the blood glucose between each group and model control group (P<0.05). In glucose tolerance test, the other groups had significant differences compared with the model control group (P<0.05). In addition, high dose of PEE-1 normal group had no significant effect on the fasting blood glucose level with normal control group (P>0.05). Results showed that PEE-1 had the hypoglycaemic function in hyperglycaemia animals, and it could be applied in the research and production of hypoglycaemic health food.