The Identification of Microbial Characteristics and Number of Colonies Isolated From River Water in the Region of Mulyoagung Singgahan TubanSupiana Dian Nurtjahyani* and dan Zainatur Robihah
Program of Biology Science Faculty of Teaching and Education PGRI University Ronggolawe Tuban, Indonesia
- *Corresponding Author:
- Supiana Dian Nurtjahyani
Program of Biology Science Faculty of Teaching
and Education PGRI University Ronggolawe Tuban, Indonesia
E-mail: [email protected]
Received Date: November 05, 2015; Accepted Date: January 07, 2016; Published Date: January 14, 2016
Citation: Nurtjahyani SD, Robihah DZ (2016) The Identification of Microbial Characteristics and Number of Colonies Isolated From River Water in the Region of Mulyoagung Singgahan Tuban. J Trop Dis 4:199. doi:10.4172/2329-891X.1000199
Copyright: © 2016 Nurtjahyani SD, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
River is one source of fresh water flowing from upstream to downstream which has a lower surface of the earth’s surface and the flow will lead to sea, lakes, swamps, and others. Nglirip River is one of the Rivers in the village of Mulyoagung Singgahan District of Tuban surrounded by the agricultural sector around the population and it is usually used by people as the fulfilment of daily domestic activities. This study aims to identify the characteristics of microbial colonies were isolated from the River of Nglirip and identify the difference in the number of microbial colonies. The dilution method used was an experimental laboratory. Data were analyzed descriptively and quantitatively with the Mann-Whitney. The result of conducted research using the pour plate method 171 cfu bacterial colonies obtained from the dilution of 10-5 and 116 cfu bacteria colonies of dilution 10-6 and after Mann-Whitney test, there was no significant difference of colonies number of microbes between dilution 10-5 and 10-6 dilutions. The conclusion is that the characteristics of microbial colonies were isolated from River water Nglirip Tuban area Mulyoagung majority are round, white colored milk and belonged to Gram-negative bacteria. And there was no significant difference in the number of colonies of microbes in different dilutions.