THE IDENTIFICATION OF RAPD AND ISSR INFORMATIVE MARKERS WITH SOME QUALITY TRAITS OF FRUIT IN SOME OF IRANIAN DATE PALM
|Monire Marsafari*1, Ali Ashraf Mehrabi2 and Zahra Tahmasebi2
|Corresponding Author: Monire Marsafari,Assistant Professor, Faculty of Agriculture, Department of Agronomy and Plant Breeding, Ilam University, Iran, E-mail: [email protected]; Tel 989163140748.|
|Received: 28 February 2014 Accepted: 05 April 2014|
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The association of 11 morphological traits with molecular markers was investigated by 24 molecular markers based on PCR (14 ISSR primers and 10 RAPD primers) in 15 cultivars of date palm collected from the south and south west of Iran. Using simple linear and multiple regressions, each of the 11 traits was regressed on all 284 available polymorphic markers (162 ISSR markers and 122 RAPD markers). A total of 89 ISSR and 84 RAPD markers gave significant associations with at least one of the 11 traits, either with linier or with multiple regression. Considerable amount of morphological changes were justified by UBC 886 primers (of ISSR primers) and Oligo 42 (RAPD marker) by polymorphism information content (PIC), 0.239 and 0.230, respectively. These results showed that based on good distribution of polymorphism inter-simple sequence repeat amplification (ISSR) and random amplification polymorphic (RAPD) in genome of date palm and the markers with high association with morphological traits, they can be used in identification of informative markers association with important morphological traits.