alexa The Impact of the Physiochemical Properties of Manufactured Nanoparticles on <em>In vitro</em> and <em>In vivo</em> Evaluation of Particle Toxicity | OMICS International| Abstract

ISSN: 2161-0398

Journal of Physical Chemistry & Biophysics

  • Mini Review   
  • J Phys Chem Biophys 2014, Vol 4(2): 139
  • DOI: 10.4172/2161-0398.1000139

The Impact of the Physiochemical Properties of Manufactured Nanoparticles on In vitro and In vivo Evaluation of Particle Toxicity

Masanori Horie1* and Hitoshi Iwahashi2
1Health Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Takamatsu, Kagawa, Japan
2Faculty of Applied Biological Sciences, Gifu University, Gifu, Japan
*Corresponding Author : Masanori Horie, Health Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 2217-14 Hayashi-cho, Takamatsu, Kagawa 761-0395, Japan, Tel: +81-87-869-4197, Email: [email protected]

Received Date: Mar 01, 2014 / Accepted Date: Apr 26, 2014 / Published Date: Apr 28, 2014

Abstract

Recently, many in vitro studies evaluating the effects of nanoparticles on cellular physiology have been reported. In in vitro systems, the nano-objects induce not only primary effects but also confounding (artificial) effects. Investigations into the physiological and pathological effects induced in cells by in vitro exposure to nano-objects may be confounded by the specific physical and chemical properties of the objects. For example, protein adsorption from the culture media to the surfaces of nano-objects can essentially starve the cells. In addition, certain nanoparticles can release metal ions into cell culture or bioassay reagents. The protein adsorption and metal ion release by the nano-objects can interfere with ELISA and LDH assays, producing inaccurate results. Moreover, unstable or non-homogenous suspensions of nano-objects can result in imprecise in vitro evaluations of nano-objects. For accurate in vitro testing of nanoparticles, we should consider the effects of these three important properties of nanosuspensions: protein adsorption, metal ion release, and suspension stability.

Keywords: Nano-object; Nanosuspension; Adsorption; Metal ion; Suspension stability

Citation: Horie M, Iwahashi H (2014) The Impact of the Physiochemical Properties of Manufactured Nanoparticles on In vitro and In vivo Evaluation of Particle Toxicity. J Phys Chem Biophys 4: 139. Doi: 10.4172/2161-0398.1000139

Copyright: © 2014 Horie M, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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