The Influence of Botanical Origin and Physico-chemical Parameters on the Antifungal Activity of Algerian Honey
- *Corresponding Author:
- Ahmed Moussa
Institute of Veterinary Sciences University Ibn-Khaldoun Tiaret (14000) Algeria
Fax: +21346 425001
E-mail: [email protected]
Received date: August 12, 2012; Accepted date: September 19, 2012; Published date: September 24, 2012
Citation: Moussa A, Noureddine D, Saad A, Abdelmalek M, Salima B (2012) The Influence of Botanical Origin and Physico-chemical Parameters on the Antifungal Activity of Algerian Honey. J Plant Pathol Microb 3:132. doi:10.4172/2157-7471.1000132
Copyright: © 2012 Moussa A, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
The purpose of the study was to characterize the physicochemical of 6 natural honeys, and to evaluate the antifungal activity of honey. Honey samples were collected from different locations of Algeria Republic. Pollen profile, colour, moisture content, ash, electrical conductivity and pH, were the parameters analyzed in each honey sample. The antifungal activity of honey samples were tested by 100% and 50% (wt per vol) concentration against Candida albicans and Rhodotorula mucilaginosa, and by the agar well diffusion method and spectrophotometric assay. Ketoconazole 2% and Nystatin (100 U), were used as positive controls.
The floral identification of honeys allowed to cluster them, as monofloral and polyfloral honeys. Concerning the physicochemical parameters, all honey samples were found to meet European Legislation (EC Directive 2001/110) for all parameters. The mean values obtained for the physico-chemical parameters were: pH 4.1; 15.31% moisture; 0.24% ash, 0.39 ms cm-1 electrical conductivity and11.95 free acidity. Inhibition zones for C. albicans (6 of 10 mm) and R. mucilaginosa (6 of 20 mm) were observed. Also, the percentage inhibition(%) for C. albicans (69.76 of 99.85) and R. mucilaginosa (83.03 of 99.77). The antifungal activity of honeys is related to their floral origin, and physicochemical properties constitute a useful resource for the generation of functional foods.