alexa The Investigation of the Oxidative Stress-Related Parameters in High Doses Methotrexate-Induced Albino Wistar Rats
ISSN: 0975-0851

Journal of Bioequivalence & Bioavailability
Open Access

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Research Article

The Investigation of the Oxidative Stress-Related Parameters in High Doses Methotrexate-Induced Albino Wistar Rats

Saka S1,2* and Aouacheri O1

1Department of Biochemistry, Badji Mokhtar University, Annaba, Algeria

2Department of Biology, Badji Mokhtar University, Annaba, Algeria

*Corresponding Author:
Saka S
Faculty of Sciences
Applied Biochemistry and Microbiology Laboratory
Department of Biochemistry
Badji Mokhtar University
Annaba, Algeria
Tel: +213 38871061
E-mail: [email protected]

Received Date: March 21, 2017; Accepted Date: March 27, 2017; Published Date: March 31, 2017

Citation: Saka S, Aouacheri O (2017) The Investigation of the Oxidative Stress- Related Parameters in High Doses Methotrexate-Induced Albino Wistar Rats. J Bioequiv Availab 9:372-376. doi: 10.4172/jbb.1000327

Copyright: © 2017 Saka S, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.



Methotrexate (MTX) is used as a chemotherapeutic agent used to treat many cancer types. Methotrexate was known for its toxic effects involving most of the body organs including liver, kidney, intestines and blood components. The aim of the present study was to investigate the antioxidant status during oxidative stress via biological, biochemical and haematological parameters in rats subjected to oral high-doses of methotrexate. Forty male albino rats were equally divided at random into four groups; the first group was the control and the other three groups were treated per os for 7 days with three different doses (1/10, 2/10 and 3/10 of an LD50) of MTX respectively. The methotrexate-treatment of rats caused critical changes in the biological, biochemical and haematological markers. Mainly, an increase in the organo-somatic indexes (organo-megaly), a decrease in urine output value (nephotoxicity) and also a perturbation in most blood components (haematoxicity) were appeared. In addition, MTX has a prooxidant effect, which was indicated by a decrease of the antioxidant parameters in different studied tissues. In view of these results, a close relationship was shown between the increasing MTX-doses and the oxidative stress intensity, characterized by perturbations in biochemical and haematological markers.


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