Habtamu Kinde, Fekadu Regassa, Mebratu Asaye and Ayana Wubie
The present study was conducted from November 2013 to April 2014 in Addis Ababa University, College of Veterinary Medicine and Agriculture, Debre Zeit, Ethiopia on the in-vitro antibacterial activities of the steam bark of Combretum molle, the leaves of Xanthium strumarium and FR1 against Staphylococcus aureus and Streptococcus agalactiae isolated from bovine clinical mastitis. The in-vitro antimicrobial test was found with encouraging results against the test bacteria. Among the plant preparations which were tested the Mean Zone of Inhibition (MZI) of Xanthium strumarium was found with the widest zone of inhibition when compared with the other two with Mean Zone of Inhibition (mm) of 20.5, 18.5, 16, 15.25, and 13 to the 10%, 5%, 2.5%, 1.25%, and 0.625% concentrations, respectively against Staphylococcus aureus. The Mean Zone of Inhibition (mm) of FR1 was found to be 23.25, 21.25, 18.75, 16.5 and 13.75 to the 10 %, 5%, 2.5%, 1.25%, and 0.625% concentrations, respectively, which was the widest MZI when compared with the other two plant extracts of similar concentrations against Streptococcus agalactiae. The plant extracts were found with dose dependent inhibition zone against both bacterial isolates. The 10% preparations of the plant extracts were comparable with the standard antimicrobial used (Gentamicin) as positive control which show better zone of inhibition than Tetracycline. The result of the present study is indicative that these herbal preparations might be considered as an alternative option for the treatment of resistant isolates of clinical bovine mastitis after studying their safety of margin in-vivo.
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