The Liver-Enriched Transcription Factors HNF-1ÃÂ±, HNF-3ÃÂ², and C/ EBPÃÂ² Contribute to the Growth Hormone-Induced Transcription of the Progranulin a Gene in Zebrafish (Danio Rerio)
- *Corresponding Author:
- Jen-Leih Wu
Institute of Cellular and Organismic Biology
Academia Sinica, Nankang, Taipei, Taiwan
- Shao-Yang Hu
Department of Biological Science and Technology
National Pingtung University of Science and Technology
No. 1, Hseufu Road, Neipu, Pingtung 912, Taiwan
Tel: 88687703202 ext. 6356
E-mail: [email protected]
Received Date: April 24, 2014; Accepted Date: May 28, 2014; Published Date: June 18, 2014
Citation: Lin YP, Chung WJ, Wu JL, Hu SY (2014) The Liver-Enriched Transcription Factors HNF-1α, HNF-3β, and C/EBPβ Contribute to the Growth Hormone-Induced Transcription of the Progranulin a Gene in Zebrafish (Danio Rerio). J Aquac Res Development 5:237 doi: 10.4172/2155-9546.1000237
Copyright: © 2014 Lin YP, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Progranulin (PGRN) is a secreted growth factor that has been implicated in diverse biological processes
including wound healing, embryo development, morphogenesis and disease. We previously showed that PGRN is induced along with IGF-1 in tilapia liver upon GH administration. In the present study, we demonstrate that the coinduction of PGRN and IGF-1 by GH administration is a common regulation in fish also presented in zebrafish. To understand the regulatory mechanism of GH-induced PGRN expression, the zebrafish PGRN promoter was isolated and analyzed. We found that a region from -2400 to -3000 within PGRN promoter is essential for GH-induced PGRN expression. A promoter competition assay showed that HNF-3 β, HNF-1 α and C/EBP β binding motifs within the -2400/-3000 region of PGRN promoter contributed to GH-induced PGRN expression. The individual or concomitant transfection of HNF-3 β, HNF-1 α and C/EBP β into HepG2 cells showed that the three transcriptional regulators all participate in GH-induced PGRN expression with independent activities. These results demonstrated that the liverenriched transcription factors HNF-3 β, HNF-1 α and C/EBP β are involved in GH-induced PGRN expression in liver. We expect that these results will be useful in understanding the regulatory relationship between PGRN and IGF-1 in response to GH regulation.