alexa The Natural Expression of Genes Encoding Major Antigens of Rd1 and Rd9 in M. Tuberculosis and other Mycobacteria | OMICS International
ISSN: 2161-1068

Mycobacterial Diseases
Open Access

Like us on:
OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article

The Natural Expression of Genes Encoding Major Antigens of Rd1 and Rd9 in M. Tuberculosis and other Mycobacteria

Shumaila N. M. Hanif*, Rajaa Al-Attiyah and Abu Salim Mustafa

Department of Microbiology, Faculty of Medicine, Kuwait University, Kuwait

Corresponding Author:
Shumaila Nida Muhammad Hanif
Department of Microbiology, Faculty of Medicine
Kuwait University, P.O.BOX 24923 13110, Safat, Kuwait
Telephone: 00965-97542085
Fax: 00965-25332719
E-mail: [email protected]

Received Date: November 28, 2011; Accepted Date: December 13, 2011; Published Date: december 15, 2011

Citation: Hanif SNM, Al-Attiyah R, Mustafa AS (2011) The Natural Expression of Genes Encoding Major Antigens of Rd1 and Rd9 in M. www.omicsonline.org/2168-958X/2168-958X-1-102.phpTuberculosis and other Mycobacteria. Mycobact Diseases 1:105. doi:10.4172/2161-1068.1000105

Copyright: © 2011 Hanif SNM, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

The natural expression of genes of five major antigenic proteins present in the genomic region of difference (RD)1, i.e. pe35, ppeE68, esxA and esxB , and RD9, i.e. esxV , was determined, at mRNA and protein levels, in M. tuberculosis, M. bovis BCG, and environmental mycobacterial species of M.avium and M.vaccae . The expression of mRNA was determined using total mycobacterial RNA and gene-specific forward and reverse primers in reverse transcription-polymerase chain reaction (RT-PCR), and the expression of proteins was determined by Western immunoblot analysis of mycobacterial sonicates with anti-sera containing protein-specific antibodies raised in rabbits. Furthermore, the expression of each protein in various sub-cellular fractions of M.tuberculosis was studied by Western immunoblot analysis of cell walls, culture filtrates and sonicates of M.tuberculosis using the rabbit anti-sera. The results showed mRNA and protein expressions for PE35, PPE68, ESXA, and ESXB only in M. tuberculosis , whereas ESXV was expressed in M.tuberculosis and M.bovis BCG, but none of them were expressed in M.avium and M.vaccae . Furthermore, all of the proteins were detected in various sub-cellular fractions of M. tuberculosis. In conclusion, our study demonstrates that the five major antigenic proteins encoded by genes present in RD1 and RD9 regions are naturally expressed in M.tuberculosis , whereas only ESXV is expressed in M. bovis BCG and none of these proteins are expressed in the environmental mycobacterial species of M. avium and M. vaccae . As these proteins are major antigens recognized by Th1 cells of protective phenotype, they could be useful in specific diagnosis and/or development of new vaccines against tuberculosis.

Keywords

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri & Aquaculture Journals

Dr. Krish

[email protected]

1-702-714-7001Extn: 9040

Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001Extn: 9042

 
© 2008- 2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version