alexa THE PATTERN OF GENE EXPRESSION UNDER WATER STRESS USING


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Research Article

THE PATTERN OF GENE EXPRESSION UNDER WATER STRESS USING CDNA-AFLP IN WHEAT (TRITICUM AESTIVUM L.)

Mona Soltani*, Amir Mohammad Naji, Alaeddin Kordenaieej
Department of Agricultural Biotechnology, Shahed University, Tehran, Iran
Corresponding Author: Mona Soltani,Department of Agricultural Biotechnology, Shahed University, Tehran, Iran, E-mail: [email protected]
Received: 04 April 2014 Accepted: 25 May 2014
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Abstract

Drought is a major environmental factor that limits the productivity of crop and other products, around the world. Plants have developed various strategies to deal with this tension that one way is to change the pattern of gene expression, due to the activation of specific genes stress and generally rewriting the genetic activity in the cells. Methods cDNA-AFLD, is a powerful tool for the analysis of gene expression related to environmental stress that was used for analysis of differences between genes in wheat Tabassi (drought tolerant) and Taifun (drought-sensitive genotype), and drought stress condition matric potential of - 10. Results showed that more than 61% of the genes examined in both cultivars, were not affected by drought, over 15% of genes in both cultivars and Taifun Tabassi under drought stress showed a change in the number and intensity of expression, and less than 12% of genes, in particular, only one digit or Taifun Tabassi had a change of expression. In total, 1,268 pieces of cDNA-AFLP, and drought stress condition showed differences. In addition, some genes showed increased or decreased expression in response to drought

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